《生命科学》 2009, 21(2): 316-319

B细胞表位定位的研究进展

王洪林1，陈明亮1，刘文毅1，杨　帆1，胡晓丰1，杨　斌1，李晋涛2*

1第三军医大学大坪医院训练队，重庆400042；2第三军医大学基础部全军免疫学研究所，重庆400038

摘　要：摘　要：B细胞抗原表位的研究对免疫原性多肽和新型疫苗分子的设计都起着指导作用，同时也有利于诊断试剂的开发以及临床疾病的诊断。本文综述了近年来实验确定和理论预测B细胞蛋白质抗原表位的常用方法，以及B细胞抗原表位分析的研究方法。
关键词：B细胞表位定位；细胞表面展示技术；肽扫描

Advances in mapping of B cell epitope

WANG Hong-lin1， CHEN Ming-liang1， LIU Wen-yi1， YANG Fan1， HU Xiao-feng1， YANG Bin1， LI Jin-tao2*

1 Training Team， Daping Hospital， Third Military Medical University， Chongqing 400042， China; 2 Institute of Immunology， College of Medicine， Third Military Medical University， Chongqing 400038， China

Abstract: Abstract:Mapping of B-cell epitope plays a guiding role on the design of immunogenic peptides and new molecular vaccine. At the same time， it is also conducive to the development of diagnostic reagents and clinical disease diagnosis. Both of the present common methods of experimental determination and theorical prediction of B cell antigen epitope and the methods of B cell epitope analysis were summarizied in this paper.
Key words: mapping of B cell epitope; cell surface display technology; pepscan