《生命科学》 2009, 21(4): 593-598

秀丽隐杆线虫(Caenorhabditis elegans)在药物筛选中的应用

杨再昌1*，杨小生2

1贵州大学化工学院，贵阳550003；2贵州省中国科学院天然产物化学重点实验室，贵阳550004

摘　要：摘　要：基于靶点的体外药物筛选操作相对简单，成本较低，但是由于药物在体内的作用并不仅仅取决于其与靶点的作用程度，吸收、分布、代谢、排泄特征和毒性均会对早期先导物能否进入临床使用产生极大的影响，因此，药物的体内筛选受到重视。本文重点综述了秀丽隐杆线虫(C.elegans)在抗衰老、抗感染药物筛选中的应用情况。秀丽隐杆线虫结构简单、易于培养和可实现高通量筛选，在未来的药物筛选中必将发挥更重要的作用。
关键词：秀丽隐杆线虫；药物；筛选

An application of Caenorhabditis elegans for drug screening

YANG Zai-chang1*， YANG Xiao-sheng2

1 Chemical Engneering College of Guizhou University， Guiyang 550003， China; 2 Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences， Guiyang 550004， China

Abstract: Abstract: It is relatively simple and cheap for drug discovery by target-based screen in vitro， but the actions of drugs in vivo do not depend fully on the interactions between drugs and targets. Major reasons preventing many early candidates reaching market are the inappropriate ADME (absorption， distribution， metabolism and excretion) properties and drug-induced toxicity. Now more attentions were paid to the methods of drug screening in vivo. In recent years， C. elegans has been widely used as a drug screening model in drug discovery. The developments of screening for drugs increasing lifespan and antagonizing microbes using C. elegans-based assays were mainly discussed in this paper. With the advantages of easily culture， simple tissue structure， and being amenable to high-throughput screening (HTS)， C. elegans may turn out to be invaluable in the development of novel screening methods in the future.
Key words: C. elegans; drug; screening