《生命科学》 2009, 21(3): 370-376

LDL亚组分研究进展

邢瑞青，许　飞，李晓颖，傅　强*

（四川大学华西基础医学与法医学院生物化学与分子生物学教研室，成都610041）

摘　要：摘　要：根据低密度脂蛋白(low density lipoprotein， LDL)颗粒的不均一性，可以利用密度梯度超速离心法和梯度凝胶电泳法将其分成若干亚组分。近年来，对于LDL亚组分分离方法的研究取得了显著进展。除对上述两种基本实验方法进行改进外，有实验室采用Western印迹法对LDL颗粒进行分离。LDL亚组分分离方法的进步，使对LDL亚组分的认识更加深入：LDL亚组分的高度不均一性、氧化易感性及电负性等不同特性与动脉粥样硬化(atherosclerosis， AS)关系密切。LDL亚组分的研究为认识动脉粥样硬化及其相关疾病提供了重要的理论依据。
关键词：LDL亚组分；密度梯度超速离心法；梯度凝胶电泳法；动脉粥样硬化

Progress on low density lipoprotein subfractions research

XING Rui-qing， XU Fei， LI Xiao-ying， FU Qiang*

(Department of Biochemistry and Molecular Biology， West China Medical School of Preclinical and Forensic Medicine， Sichuan University， Chengdu 610041， China)

Abstract: Abstract: LDL can be divided into several subfractions by density gradient ultracentrifugation and gradient gel electrophoresis according to its heterogeneity. Several novel methods to identification of LDL subfractions have been developed recently. Except for the improvement of the two above-mentioned basic experimental methods， there is another method， i.e，Western blotting. The development of separation methods for LDL subfractions promotes the cognition of their characteristics: the highly heterogeneity， affectability to oxidation and electronegativity of LDL subfractions are closely related to atherogenesis. The theory of LDL subfractions provides important theoretical evidences for understanding of atherosclerosis and diseases related to atherosclerosis.
Key words: LDL subfractions; density gradient ultracentrifugation; gradient gel electrophoresis; atherosclerosis