《生命科学》 2009, 21(1): 156-162

原子力显微镜在细胞弹性研究中的应用

叶志义*，范　霞

重庆大学生物工程学院，重庆400044

摘　要：摘　要：细胞表面的力学性质会随着细胞所处环境的不同而发生改变，它的变化间接反映出胞内复杂的生理过程。原子力显微镜(atomic force microscope，AFM)能以高的灵敏度和分辨率检测活体细胞，通过利用赫兹模型分析力曲线可以获得细胞的弹性信息。本文简介了原子力显微镜的工作原理与工作模式，着重介绍利用AFM力曲线检测细胞弹性的方法及其在细胞运动、细胞骨架、细胞黏附、细胞病理等方面的应用成果，表明AFM已经成为细胞弹性研究中十分重要的显微技术。
关键词：原子力显微镜; 力曲线; 细胞弹性; 杨氏模量

Application of atomic force microscopy in cell elasticity

YE Zhi-yi*， FAN Xia

College of Bioengineering， Chongqing University， Chongqing 400044， China

Abstract: Abstract: Mechanical properties of living cells vary with the surrounding around， which is indirectly reflects the complex physiological processes in the cell. The atomic force microscope (AFM) can be used to probe the Young抯 modulus of living cells with high sensitivity and resolution. Hertze model is used to analyse the cell force curve. The review introduces AFM and the method of probing the elasticity of cell by force curves and its application in locomotion， adhesion， cytoskeleton as well as cell pathology and so on. All these shows that AFM has been an important technology in the research of cell elasticity.
Key words: atomic force microscopy; force- curve; cell elasticity; Young{$39}s modulus