《生命科学》 2025, 37(8): 1041-1053

基于环状单链DNA的医学合成生物学

洪明明¹ , 唐林林^2,* , 宋　杰^2,*

¹浙江工业大学药学院，杭州 310014 ²中国科学院杭州医学研究所，杭州 310022

摘　要：

随着生物医学技术的发展，双链质粒作为最常用的分子工具，已无法完全满足医学合成生物学的发展需要。环状单链DNA (circular single-stranded DNA, CssDNA) 不但具备传统质粒的基因载体功能，而且还具有低免疫原性、低整合风险且结构可编程性的特性，在未来有望成为医学合成生物学领域重要的分子工具。本文综述了CssDNA 的天然来源以及人工合成方法、生物学效应以及在医学合成生物学研究领域的应用。

通讯作者：唐林林 , Email:tanglinlin@him.cas.cn 宋　杰 , Email:songjie@him.cas.cn

Medical synthetic biology based on circular single-stranded DNA

HONG Ming-Ming¹ , TANG Lin-Lin^2,* , SONG Jie^2,*

¹College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, China ²Hangzhou Institute of Medicine, Chinese Academy of Sciences, Hangzhou 310022, China

Abstract:

The advancement of biomedical technology has revealed that double-stranded plasmids, one of the most commonly utilized molecular tools, are inadequate for fully meeting the developmental requirements of medical synthetic biology. Circular single-stranded DNA (CssDNA) not only possesses the function of a gene carrier like traditional plasmids, but also exhibits the characteristics of low immunogenicity, minimal integration risk, and structural programmability. It is expected to become a significant molecular tool in the field of medical synthetic biology in the future. Here, we comprehensively review the natural sources, synthetic methods, biological effects of CssDNA, as well as their applications in the field of medical synthetic biology.

Communication Author：TANG Lin-Lin , Email:tanglinlin@him.cas.cn SONG Jie , Email:songjie@him.cas.cn