《生命科学》 2025, 37(8): 1007-1019

RNA响应的合成基因线路设计及其生物医学应用

舒文杰 , 王　福^*

西安交通大学基础医学院，生物物理系，医学工程研究所，西安 710061

摘　要：

合成基因线路是指利用合成生物学的方法对调控元件进行设计与组装，以创造出能够执行特定生物功能的系统。近年来，各类RNA 分子响应的基因线路不断被报道，并在基础生物学研究和医疗健康领域显现出极大应用潜力。这些RNA 分子响应的基因线路具有良好的模块化和可编程特征，能够精确地执行设定任务。本文综述了近年来使用不同策略构建的各类RNA 分子响应的基因线路系统并探讨了其生物医学应用，为以后理性设计RNA 响应的基因线路提供新的思路。

通讯作者：王　福 , Email:wangfu@xjtu.edu.cn

RNA-responsive gene circuits and biomedical applications

SHU Wen-Jie , WANG Fu^*

Institute of Medical Engineering, Department of Biophysics, School of Basic Medicine, Xi'an Jiaotong University, Xi'an 710061, China

Abstract:

Synthetic gene circuits refer to the use of synthetic biology methods to design and assemble regulatory elements to create systems capable of performing specific biological functions. In recent years, various RNAresponsive gene circuits have been reported, and have shown great application potential in basic biological research and medical health. These genetic circuits are modular and programmable, enabling them to perform set tasks precisely. In this work, we review the latest reported RNA-responsive gene circuits constructed by different strategies and discuss their biomedical applications, which will provide new insights for rational design of related gene circuits in the future.

Communication Author：WANG Fu , Email:wangfu@xjtu.edu.cn