《生命科学》 2025, 37(8): 936-946
基因工程细菌传感器在肠道疾病检测中的研究进展
摘 要:
许多肠道疾病的发生和发展是一个动态演变的过程,具有长期性和反复性的特点。传统的内窥镜检查难以实现肠道疾病的早期无创诊断,而体外粪便分析通常无法反映肠道内的真实情况。利用合成生物学技术改造细菌,创制能够感知和响应特定疾病信号的基因工程细菌传感器,为人类疾病的无创式检测提供了独特的解决方案。这些工程细菌传感器被部署在哺乳动物肠道时,能够充当“哨兵”,实现肠道疾病及其相关分子标志物的非侵入式在体原位检测。本文介绍了用于肠道疾病相关分子标志物检测的细菌传感器设计原理,综述了工程细菌传感器在肠道疾病检测中的研究进展,最后对其未来的研究和应用方向进行了展望。
通讯作者:周 英 , Email:zhouying@ecust.edu.cn 叶邦策 , Email:bcye@ecust.edu.cn
Abstract:
The occurrence and development of intestinal diseases are a dynamic evolutionary process characterized by long-term and recurrent features. Traditional endoscopic examinations struggle to achieve early, non-invasive diagnoses of intestinal diseases, and stool analysis often fails to reflect the true conditions within the intestines. Employing synthetic biology techniques to engineer bacteria into genetically modified bacterial sensors that can detect and respond to specific disease signals offers a novel approach to non-invasive diagnosis of human diseases. When deployed in the mammalian gut, these engineered bacterial sensors function as 'sentinels' for the non-invasive in situ detection of gut diseases and their associated molecular biomarkers. This paper elucidates the design principles of bacterial sensors for the detection of molecular biomarkers associated with gastrointestinal diseases, reviews the advancements in research on engineered bacterial sensors for gut disease detection, and offers a discussion on future research avenues and potential applications.
Communication Author:ZHOU Ying , Email:zhouying@ecust.edu.cn YE Bang-Ce , Email:bcye@ecust.edu.cn