《生命科学》 2025, 37(7): 844-853

同步辐射红外谱学在生命科学领域中的应用

钟佳佳 , 周晓洁 , 俞文杰 , 谢鑫成 , 唐雨钊^*

中国科学院上海高等研究院国家蛋白质科学研究(上海)设施，上海 201210

摘　要：

同步辐射傅里叶变换红外光谱(synchrotron radiation Fourier transform infrared spectroscopy, RFTIR)技术具有高空间分辨率的特性，在微米尺度的结构表征上有明显优势，被广泛用于生命科学领域的研究。全球多条红外光束线站在发展SR-FTIR 显微谱学技术的同时积极推动原位及联用技术、纳米红外光谱技术、远红外/ 太赫兹技术的发展。国家蛋白质科学研究( 上海) 设施的同步辐射红外线站(BL01B) 具有优异的实验能力，自运行开放以来发展了红外微流控系统、蛋白质溶液高压构象分析系统、时间分辨停流红外光谱等多种原位技术及实验方法学，支撑了生命科学领域用户成果的持续产出。

通讯作者：唐雨钊 , Email:tangyuzhao@sari.ac.cn

Applications of synchrotron radiation infrared spectroscopy in the field of life sciences 

ZHONG Jia-Jia , ZHOU Xiao-Jie , YU Wen-Jie , XIE Xin-Cheng , TANG Yu-Zhao^*

National Facility for Protein Science in Shanghai, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China

Abstract:

Synchrotron radiation Fourier transform infrared spectroscopy (SR-FTIR) technology, characterized by its high spatial resolution, has significant advantages in the characterization of microscale structures. This technology has been widely applied in the field of life sciences. Numerous infrared beamlines around the world are not only developing SR-FTIR microspectroscopy technique but also actively promoting the development of in situ techniques, nano-FTIR spectroscopy, and far-FTIR/THz technology. The BL01B beamline at the national facility for protein science in Shanghai boasts excellent performance. Various in situ techniques and experimental methodologies have been developed, such as infrared microfluidic system, high-pressure conformation analysis system for protein solutions, and time-resolved stopped-flow IR spectroscopy. These techniques have significantly supported the research achievements of users in the field of life sciences.

Communication Author：TANG Yu-Zhao , Email:tangyuzhao@sari.ac.cn