《生命科学》 2024, 36(10): 1250-1259

基于合成生物学改造蓝细菌的光合碳固定

汪　浩^1,2,3 , 张芬芳^1,2,3 , 陈　磊^1,2,3 , 孙　韬^1,3,4,* , 张卫文^1,2,3,4,*

¹天津大学化工学院，合成微生物学实验室，天津 300072 ²教育部系统生物工程重点实验室，天津 300072 ³教育部合成生物学前沿科学中心，天津 300072 ⁴天津大学生物安全战略研究中心，天津 300072

摘　要：

蓝细菌是一类以CO2 为碳源的光能自养微生物。近年，合成生物学技术的跨越式发展使得深度理解蓝细菌光合固碳机制、提升蓝细菌光合效率成为可能。本文首先介绍目前蓝细菌中主要的模式底盘和其遗传改造工具；随后讨论蓝细菌固碳机制和通过缩短捕光天线、重塑卡尔文- 本森- 巴萨姆(Calvin-Benson-Bassham) 循环等提升光合固碳效率将CO2 转化为高价值化学品的相关研究；最后展望通过合成生物学方法对蓝细菌进行全方位改造，培育出光合固碳效率足够高，既能优化环境又具备大规模工业化生产价值的“超级”蓝细菌。

通讯作者：孙　韬 , Email:tsun@tju.edu.cn 张卫文 , Email:wwzhang8@tju.edu.cn

Modifying the photosynthesis and carbon fixation of cyanobacteria based on synthetic biology

WANG Hao^1,2,3 , ZHANG Fen-Fang^1,2,3 , CHEN Lei^1,2,3 , SUN Tao^1,3,4,* , ZHANG Wei-Wen^1,2,3,4,*

¹Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, China ²Key Laboratory of Systems Bioengineering, Ministry of Education of China, Tianjin 300072, China ³Frontier Science Center for Synthetic Biology, Tianjin 300072, China ⁴Center for Biosafety Research and Strategy, Tianjin University, Tianjin 300072, China

Abstract:

Cyanobacteria are photoautotrophic microorganisms that utilize CO2 as their carbon source. Recent advancements in synthetic biology have opened avenues for comprehending cyanobacterial photosynthesis and carbon fixation mechanisms while enhancing their efficiency in these processes. We present an overview of primary model chassis and genetic modification tools, followed by discussions on cyanobacterial photosynthesis and carbon fixation mechanisms. We further examine efforts to transform cyanobacteria for CO2 conversion into high-value chemicals, culminating in an exploration of synthetic biology's potential to enhance cyanobacterial CO2 utilization efficiency. Ultimately, the comprehensive transformation of cyanobacteria via synthetic biology methods holds promise for cultivating "super" cyanobacteria chassis with heightened photosynthetic and carbon fixation efficiencies, thereby optimizing environments and unlocking substantial industrial production value.

Communication Author：SUN Tao , Email:tsun@tju.edu.cn ZHANG Wei-Wen , Email:wwzhang8@tju.edu.cn