《生命科学》 2023, 35(7): 966-976

多能干细胞来源的内皮细胞研究及应用进展

雷　佳 , 杨　隽^*

浙江大学医学院附属第二医院生理科、心内科，杭州 310058

摘　要：

内皮细胞(endothelial cells, ECs) 为血管的内侧单层细胞，能够维持血管壁完整性与通透性、调节血管张力、参与细胞黏附。在研究疾病机制及再生医学中都需要大量功能性的内皮细胞，仅依靠从人体内分离的办法很难获得大量可应用的功能性内皮细胞。相比之下，多能干细胞(pluripotent stem cells, PSCs) 可以进行高效诱导分化成内皮细胞，为解决当前建立包含特异性血管内皮的不同组织体外长期培养需要大量内皮细胞的难题提供方案。在此，该文总结了利用多能干细胞定向诱导产生的内皮细胞在基础研究和疾病治疗中的最新应用进展，为组织芯片、类器官构建等临床转化研究提供新思路。

通讯作者：杨　隽 , Email:yang_jun@zju.edu.cn

Advances in research and application of endothelial cell derived from pluripotent stem cells

LEI Jia , YANG Jun^*

Department of Physiology and Department of Cardiology of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310058, China

Abstract:

Endothelial cells (ECs) are the inner monolayer cells of blood vessels, which maintain the integrity and permeability of blood vessel wall, regulate the tension of blood vessel and participate in cell adhesion. Functional endothelial cells are necessities in the study of disease mechanisms and regenerative medicine. To obtain a large number of functional endothelial cells by isolation from human tissue is rather difficult. In contrast, pluripotent stem cells (PSCs) are feasible to be efficiently differentiated into endothelial cells, solving the current dilemma of supplying a large amount of ECs for setting up tissue culture with selective endothelial barrier for long term culture. In this review, we describe how endothelial cells induced from pluripotent stem cells be used in basic research and disease treatment, and provide advanced thoughts for the translational study such as tissue chips, organ culture etc.

Communication Author：YANG Jun , Email:yang_jun@zju.edu.cn