《生命科学》 2022, 34(7): 863-870

肺芯片的构建及其在肺疾病中的仿生应用

李逸轩^1,2 , 杨　爽^1,2 , 朱　彦^1,2 , 毛红菊^3,* , 杨　剑^1,2,*

¹天津中医药大学，国家组分中药重点实验室，天津 301617 ²天津中医药大学，天津市中药药理学重点实验室，天津 301617 ³中国科学院上海微系统与信息技术研究所，传感技术联合国家重点实验室，上海 200050

摘　要：

随着微工程的快速发展，多学科交叉产物— 微流控器官芯片以其能高度模拟体内微环境、便捷测定生理参数等优点，成为最具价值的动物替代模型之一。新冠疫情肆虐，使得肺芯片的构建及应用越来越广泛，其高度模拟了体内肺组织微环境，包括可溶性细胞因子的表达和细胞- 细胞间、细胞- 细胞外基质间的相互作用，通过体外诱导及给药可探究多种相关细胞因子的表达，达到快速筛药的目的。该文主要阐述了两种类型的肺芯片，即肺泡芯片和小气道芯片，总结其在肺疾病的仿生应用和快速药物筛选中的现况，期望为肺芯片的精准、广泛使用提供有效的科学定位和依据。

通讯作者：毛红菊 , Email:hjmao@mail.sim.ac.cn 杨　剑 , Email:wosyjianya@126.com

Construction of a pulmonary organ chip and its bionic applications

LI Yi-Xuan^1,2 , YANG Shuang^1,2 , ZHU Yan^1,2 , MAO Hong-Ju^3,* , YANG Jian^1,2,*

¹State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China ²Tianjin Key Laboratory of Pharmacology of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China ³State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China

Abstract:

With the rapid development of micro-engineering technologies, organs-on-chips as the multi-disciplinary product, has become one of the most valuable animal alternative models. It highly simulates the micro-environment in vivo and the physiological parameters can be accurately measured. The construction and application of lung chips are increasingly extensive with the spread of COVID-19. It highly mimics the micro-environment of lung tissue in vivo, including the expression of specific soluble cytokines, cell-cell and cell-extracellular matrix interactions. Through the stimulus induction and drug administrations on the chips, we can achieve the purpose of rapid drug screening via testing the expression of a variety of related cytokines. Thus, this article mainly describes two types of lung chips—alveolar chips and small airway chips, and summarizes the current status of their applications in lung disease bionics and rapid drug screening, so as to present the scientific evidence regarding the accurate and widespread use of lung chips.

Communication Author：MAO Hong-Ju , Email:hjmao@mail.sim.ac.cn YANG Jian , Email:wosyjianya@126.com