《生命科学》 2019, 31(7): 731-738

CRISPR/Cas9基因编辑技术在前列腺癌研究中的进展

胡　坤1，刘雨函2，肖俊文2，廖新惠2，陈杰青2，张仲富2，吴建挺2，梅红兵1，2*

(1 安徽医科大学深圳市第二人民医院临床医学院泌尿外科，深圳 518000；2 深圳市第二人民医院泌尿外科，深圳大学第一附属医院泌尿外科，深圳市泌尿生殖系统肿瘤研究重点实验室， 深圳 518000)

摘　要：摘　要：CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9) 基因编辑技术是基于细菌和古细菌适应性免疫防御系统发展而来的一种基因编辑工具。Cas9蛋白借助gRNA的引导靶向目标基因进而实现对目标基因精确、高效的编辑。因其具有操作简单、成本低的特点，被研究人员广泛应用，并在前列腺癌相关基因及信号通路的研究中发挥着重要作用。该文将对CRISPR/Cas9基因编辑技术的发展、作用机理及其在前列腺癌研究中的应用进展进行综述。

Progress of CRISPR/Cas9 gene editing technology in prostate cancer

HU Kun1， LIU Yu-Han2， XIAO Jun-Wen2 ， LIAO Xin-Hui2， CHEN Jie-Qing2， ZHANG Zhong-Fu2， WU Jian-Ting2， MEI Hong-Bing1，2*

(1 Urology Department， Shenzhen Second People′s Hospital， Clinical Medicine College of Anhui Medical University， Shenzhen 518000， China; 2 Key Laboratory of Medical Reprogramming Technology， Shenzhen Second People’s Hospital， The First Affiliated Hospital of Shenzhen University， Shenzhen518000， China)

Abstract: Abstract: CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9) technology is a gene editing tool based on the development of bacterial and archaeal adaptive immune defense systems. The Cas9 protein targets the gene by gRNA guidance to achieve accurate and efficient editing of the target genes. Because of its simple operation and low cost， it is widely used and plays an important role in the research of prostate cancer related genes and signaling pathways. Here， we briefly introduce the development process and mechanism of the CRISPR/Cas9 as well as summarize its application in the research of prostate cancer.