《生命科学》 2018, 30(9): 987-993

基因编辑技术在莱茵衣藻中的应用进展

涂文凤1，王　月1，2，杨文强1*

(1 中国科学院植物研究所光生物学重点实验室，北京 100093；2 中国科学院大学研究生院，北京 100049)

摘　要：摘　要：基因编辑技术已经成为功能基因组学和作物分子育种精准且有力的工具。莱茵衣藻(Chlamydomonas reinhardtii，简称衣藻) 是光合作用、黑暗异养代谢、厌氧代谢和生物制氢、营养和能量代谢等研究领域的重要模式生物。近20 年来，基因编辑技术，如锌指核酸酶(ZFNs)、转录激活物样效应物核酸酶(TALENs)、CRISPR/Cas9 和CRISPR/Cpf1 等的发展，更加推动了以衣藻为模式生物的研究。现对衣藻中的核基因组靶向基因编辑技术的应用及最新研究进展等进行总结，以期为衣藻相关领域的研究提供参考。

Progress in the application of genome editing in Chlamydomonas reinhardtii

TU Wen-Feng1， WANG Yue1，2， YANG Wen-Qiang1*

(1 Key Laboratory of Photobiology， Institute of Botany， Chinese Academy of Sciences， Beijing 100093， China; 2 School of Graduate， University of Chinese Academy of Sciences， Beijing 100049， China)

Abstract: Abstract: Genome editing has been working as precise and powerful tools in the studies of functional genomics and crop breeding. Chlamydomonas reinhardtii is an important model organism in the fields of photosynthesis， dark heterotrophic metabolism， fermentative metabolism and biohydrogen production， nutrients and energy metabolisms， and etc. In the last two decades， the development of genome editing techniques， such as zinc-finger nucleases (ZFN)， transcription activator-like effectors nuclease (TALEN)， clustered regularly interspaced short palindromic repeat (CRISPR)/associated protein 9 (Cas9) and CRISPR/Cpf1， has promoted researches on Chlamydomonas. In this review， we summarized the application and progress of genome editing techniques targeting Chlamydomonas nucleus encoded genes， aiming to provide references to Chlamydomonas studies.