《生命科学》 2018, 30(9): 950-954

非人灵长类基因修饰模型构建技术研究进展

刘　真，廖兆蒂，孙　强*

(中国科学院神经科学研究所，中国科学院脑科学与智能技术卓越创新中心，
    神经生物学国家重点实验室，中国科学院灵长类神经生物学重点实验室，上海 200031)

摘　要：摘　要：随着以CRISPR-Cas9 为代表的新型基因编辑技术的出现及发展应用，科学家们在构建非人灵长类基因修饰模型方面取得了很多重要进展。然而，首建动物嵌合、复杂基因修饰低效、传代周期长以及遗传背景复杂等多方面因素极大地限制了非人灵长类基因修饰模型的开发和应用。现对目前非人灵长类基因编辑模型构建的进展进行综述，并重点对基因编辑技术结合体细胞核移植、精原干细胞、胚胎干细胞及单倍体干细胞等技术手段在非人灵长类基因修饰模型构建中的进展进行综述和展望。

Advance of technologies in generating gene-modified non-human primates

LIU Zhen， LIAO Zhao-Di， SUN Qiang*

(State Key Laboratory of Neuroscience， Key Laboratory of Primate Neurobiology， CAS Center for Excellence in Brain Science and Intelligence Technology， Institute of Neuroscience， Chinese Academy of Sciences， Shanghai 200031， China)

Abstract: Abstract: With the recently developed gene-editing methods， such as CRISPR-Cas9， significant progresses have been achieved in the generation of gene-modified non-human primates (NHP). However， the development and application for gene-modified NHP are still limited due to mosaicism in founder animals， low efficiency in complex gene modification， long sex mature time in passage and complicated genetic background. Here we first review the recent achievements in the generation of gene-modified NHP. Then， we focus the development and advantage of somatic cell nuclear transfer， spermatogonia stem cell， embryonic stem cell and haploid stem cell in the generation of gene-modified NHP models.