《生命科学》 2017, 29(10): 1000-1006

诱导重编程的表观遗传调控研究进展

李东伟，陈捷凯，裴端卿*

(中国科学院广州生物医药与健康研究院再生生物学中国科学院重点实验室，干细胞与再生医学广东省重点实验室，广州 510530)

摘　要：摘　要：表观遗传调控是细胞命运变化与决定的重要基础之一。2006 年，日本科学家山中伸弥发现通过4个转录因子Oct4、Sox2、Klf4 和c-Myc 可以将已经分化的体细胞逆转回与胚胎干细胞相似的多能性状态，获得诱导多能干细胞(induced pluripotent stem cells， iPSCs)。这种诱导重编程技术不仅是干细胞技术的一大突破，也提供了关键的体外模型用于研究细胞重编程的表观遗传机制。对该机制的深入理解将推动人类自由操纵细胞命运的进程，从而有望治疗各种因功能细胞、组织、器官缺失退化引发的疾病。从诱导重编程的表观遗传调控方向的研究进展出发，阐述通过诱导重编程发现的关键细胞命运转变表观调控机制，展望未来的主要研究目标。

Progress in epigenetic regulation mechanism of induced reprogramming

LI Dong-Wei， CHEN Jie-Kai， PEI Duan-Qing*

(CAS Key Laboratory of Regenerative Biology， Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine， Guangzhou Institutes of Biomedicine and Health， Chinese Academy of Sciences， Guangzhou 510530， China)

Abstract: Abstract: Epigenetic regulation is one of the fundamental mechanisms of cell fate transition and determination. In 2006， Japanese scientist Shinya Yamanaka found that differentiated somatic cells can be reprogrammed to embryonic stem cell-like pluripotent state， named induced pluripotent stem cells (iPSCs) by induction of four transcriptional factors including Oct4， Sox2， Klf4 and c-Myc. This induced programming technique is not only a breakthrough on stem cells field， but also opens a gate for studying epigenetic regulation mechanism of cellular reprogramming in vitro. Understanding this mechanism will push the process of cell fate manipulation， then take advantage on regenerative medicine to treat disease caused by injury， degeneration or defect of functional cells， tissue or organ. Here we reviewed the progress in epigenetic regulation mechanism of induced reprogramming and gave a perspective of future research aim in this field.