《生命科学》 2010, 22(9): 901-905

哺乳动物精子冷冻的抗氧化研究进展

师　红1，文瑞丽1，马清义2，金学林2，吴民耀1*

(1 陕西师范大学生命科学学院，西北濒危药材资源开发国家工程实验室，西安 710062；2 陕西省珍稀野生动物抢救饲养研究中心，西安 710062)

摘　要：摘　要：人工授精是迄今为止应用最广泛并最有成效的辅助生殖技术，而高品质的精液是提高人工授精受胎率的关键。近年来在家畜精液冷冻保存技术中应用抗氧化剂的研究受到广泛关注，通过添加抗氧化剂降低了精子在冷冻保存过程中所遭受的氧化损伤，提高了冷冻精液质量和母畜的受胎率。可添加的抗氧化剂种类很多，通常有维生素类和酶类抗氧化剂等。针对目前抗氧化剂在大熊猫精液上应用研究甚少的现状，该文对哺乳动物精子的氧化损伤机制和常用的抗氧化剂进行综述，期望对大熊猫的相关研究提供理论依据和参考。
关键词：冷冻精子；抗氧化剂；大熊猫

Recent research on antioxidation of mammal frozen sperm

SHI Hong1， WEN Rui-li1， MA Qing-yi2， JIN Xue-lin2， WU Min-yao1*

(1 National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China， College of Life Science， Shaanxi Normal University， Xi抋n 710062， China; 2 Shaanxi Province Rare Wildlife Rescue Center， Xi抋n 710062， China)

Abstract: Abstract: So far， artifical insemination is the most widely used and effective assisted-reproductive technology， and it is crucial for improving the rate of artificial insemination pregnancy to acquire the high-qaulity semen.Recently， the research on the application of antioxidants to livestock semen cryopreservation is widely concerned. By adding antioxidants to the semen， it can reduce oxidative damage of sperm during cryopreservation process， improve the quality of frozen semen and the pregnancy rate of female livestock. There are many kinds of antioxidants can be used， such as vitamins， enzymes， and so on. Since the application of antioxidants to giant panda semen is seldomly reported， in order to provide a theoretical basis to related research of giant panda， we make a review on the mechanism of oxidative damage of mammalian sperm and the antioxidants used frequently.
Key words: frozen sperm; antioxidants; giant panda