《生命科学》 2010, 22(8): 817-822

原子力显微镜研究细胞弹性的数据分析方法

叶志义*， 张　丽

（重庆大学生物工程学院生物流变科学与技术教育部重点实验室， 重庆 400044）

摘　要：摘　要：细胞的力学性质与生命体的功能和健康都是息息相关的，而原子力显微镜(atomic force microscope，AFM)是研究细胞力学性质最好的仪器之一。该文较为详细叙述了AFM力曲线测量原理，以及分析AFM实验数据的常用模型。作者用该文所提到的模型分析了肺癌细胞的弹性，结果显示在加载速率比较低的情况下，三种计算方法计算得到的结果没有显著差别，而在加载速率高于8 mm/s时，结果则有显著差异。
关键词：原子力显微镜；力曲线；杨氏模量；模型

Elasticity measurement of cells with AFM: the data processing

YE Zhi-yi*， ZHANG Li

(Key Laboratory of Biorheological Science and Technology， Ministry of Education， Bioengineering College， Chongqing University， Chongqing 400044， China)

Abstract: Abstract: Mechanical properties of individual living cells which can be probed by AFM， one of the best tools， are known to be closely related to both health and function of the human body. Measurement principle of force curve of AFM， as well as several models applied to analyze the experimental data， is introduced in this review. Furthermore， elasticity of lung cancer cells is calculated according to the models proposed in this paper， which indicates that corresponding Young{$39}s moduluses are approximate under slow loading rate， but different significantly when loading rate is greater than 8 mm/s.
Key words: AFM; force curve; Young{$39}s modulus; model