《生命科学》 2010, 22(1): 64-68

人羊膜上皮细胞和胚胎干细胞

赖东梅^1*， 郭礼和²

(1上海交通大学附属国际和平妇幼保健院，上海200030；2和泓生物技术（上海）有限公司，上海201203)

摘　要：摘　要：人胚胎干细胞有着巨大的医学应用前景，但人胚胎干细胞要求的生长条件很高，体外很难模拟其生长的体内环境，因此控制人胚胎干细胞的生长常不理想，而使用鼠胚胎成纤维细胞(MEF)作为滋养层则存在动物源性污染的问题。该文阐述人羊膜上皮细胞(HAEC)的特点及其作为滋养层培养胚胎干细胞的现状，并探讨基因组 DNA甲基化修饰在胚胎干细胞分化过程中的作用，为建立更优化的培养系统提供依据。
关键词：人羊膜上皮细胞；胚胎干细胞；表观修饰

Human amniotic epithelial cells and embryonic stem cells

LAI Dong-mei^1*， GUO Li-he²

(1 The International Peace Maternity and Child Health Hospital， Shanghai Jiao Tong University， Shanghai 200030， China; 2 Hehong (Shanghai) Biotechnology Ltd， Shanghai 201203， China)

Abstract: Abstract: Human embryonic stem cells have great prospects for medical applications， but the growth of human embryonic stem cells requires high quality of culturing conditions in vitro and it is difficult to simulate in vivo environment， therefore the control of human embryonic stem cells?growth is often not ideal. The use of mouse embryonic fibroblasts (MEF) as feeder layer exists the problem of pollution of animal pathogens. In this paper， the characteristics of human amniotic epithelial cells (HAEC) and its use as a feeder layer of embryonic stem cells are described. The genomic DNA methylation in embryonic stem cell differentiation is also explored. Optimization and standardization of culture methods is needed for research as well as for clinical purpose.
Key words: human amniotic epithelial cells; embryonic stem cells; epigenetic modification