疾病特异诱导多能干细胞研究进展
王译萱，蒋永华，高绍荣*
(北京生命科学研究所，北京102206)
摘 要：
摘 要：通过病毒或非病毒转导体系，在小鼠和人的体细胞中人为表达几个与细胞多能性相关的转录因子，从而使细胞达到类似于胚胎干细胞(embryonic stem cells， ESCs)状态，是近年来新发展起来的体细胞重编程技术。这些被重编程的细胞称为诱导多能干细胞(induced pluripotent stem cells， iPS细胞)。这项技术为获得患者和疾病特异的多能干细胞提供了新的途径。患者和疾病特异的iPS细胞的获得，不仅在避免免疫排斥的宿主特异的细胞移植治疗上有广泛前景，并对了解疾病发生机理、药物筛选和毒性研究有着重要的意义。该文综述从iPS细胞技术的发明入手，着重讨论疾病iPS细胞的研究进展及其在应用于治疗时亟需解决的问题。
关键词：干细胞；iPS细胞；疾病iPS细胞；重编程；Oct3/4；Sox2；c-Myc；Klf4
中图分类号：Q813 文献标识码：A
Advances in generation of disease-specific induced pluripotent stem (iPS) cells
WANG Yi-xuan， JIANG Yong-hua， GAO Shao-rong*
(National Institute of Biological Sciences， Beijing 102206， China)
Abstract:
Abstract: A recently developed reprogramming techonology can reprogram mouse and human somatic cells to an undifferentiated state similar to that of embryonic stem (ES) cells by viral or nonviral transduction of several specific transcription factors， and the reprogrammed cells are termed induced pluripotent stem (iPS) cells. This technology has opened a new avenue to generate patient-and disease-specific pluripotent stem cells， which are useful not only for customized cell transplantation therapy without immune rejection but also for disease mechanisms understanding， drug screening and toxicology study. In this review， we will discuss the overview of the development of iPS cells techonology， and focus on the research progress of disease-specific iPS cells and the problems need to resolve in clinical research.
Key words: stem cell; iPS cells; disease correlated iPS cells; reprogramming; Oct3/4; Sox2; c-Myc; Klf4
