《生命科学》 2009, 21(3): 467-471

电穿孔基因导入技术在临床前与临床试验中的应用

栾好飞1，2，付　洁1，宋海峰1*

（1 军事医学科学院放射与辐射医学研究所，北京100850；2 安徽医科大学基础医学院，合肥230032）

摘　要：摘　要：电穿孔基因作为非病毒递送手段之一，可安全、有效地将外源核酸导入靶组织或器官。近年来用电穿孔法进行基因治疗的报道不断增多，优势日趋显著。本文重点阐述了电穿孔在基因治疗中的优势、影响因素及在临床前和临床试验中应用。
关键词：电穿孔；基因治疗；临床前研究；临床试验

Application of gene introduction in vivo by electroporation in preclinical and clinical trials

LUAN Hao-fei1，2， FU Jie1， SONG Hai-feng1*

(1 Institute of Radiation Medicine， Academy of Military Medical Sciences， Beijing 100850， China; 2 College of Preclinical Medicine， Anhui Medical University， Hefei 230032， China)

Abstract: Abstract：The electroporation can delivery the exogenous nucleic acid into the target tissue or the organ effectively and safely as one of the non-virus delivery methods. In recent years， electroporation has been adopted in the gene therapy studies with significant increasingly advantages. This article mainly elaborated the superiority of electroporation in gene therapy and the influencing factors， as well as the applications in the preclinical and clinical trial.
Key words: electroporation ; gene therapy; preclinical trial; clinical trial