《生命科学》 2009, 21(1): 15-20

小角X-射线散射法解析多结构域蛋白质或复合物的溶液结构

刘　帅，胡红雨*

中国科学院上海生命科学研究院生物化学与细胞生物学研究所，上海200031

摘　要：摘　要：随着同步辐射装置的建设与发展及各种建模方法的产生与完善，小角X-射线散射(small angle X-ray scattering， SAXS)法已经逐渐成为结构生物学中的一种重要的工具。SAXS可以用于研究溶液中生物大分子的结构及构象变化，蛋白质的组装、折叠等动态过程。本文对SAXS的基本原理、常用的研究技术和建模方法及其应用进行了综述。
关键词：小角X-射线散射；多结构域蛋白质；蛋白质复合物；从头计算；刚体建模

Elucidating the solution structures of multi-domain proteins and complexes by small angle X-ray scattering

LIU Shuai， HU Hong-yu*

State Key Laboratory of Molecular Biology， Institute of Biochemistry and Cell Biology， Shanghai Institutes for Biological Sciences， Chinese Academy of Sciences， Shanghai 200031， China

Abstract: Abstract: Small angle X-ray scattering (SAXS) is becoming an important tool in structural biology， as the development of synchrotron radiation facilities and various modeling methods. SAXS can be applied to determine the solution structures of multi-domain proteins， protein complexes and their conformational changes. It can also be used to study the dynamic processes of protein folding and assembly. In this review， we survey the basic theory， techniques， modeling and application of SAXS.
Key words: small angle X-ray scattering; multi-domain protein; protein complex; ab initio; rigid body modeling