《生命科学》 2008, 20(4):

小动物体内可见光三维成像技术研究进展

杨华瑜1，韩　彧2，董洪莹2，赵春林2*

(1 中国医学科学院 中国协和医科大学 北京协和医院 肝脏外科， 北京 100730；2 北京龙脉得-冷泉港生物科技股份有限公司， 北京 100037)

摘　要：摘　要：活体动物体内可见光成像是采用生物发光和荧光为标记物，利用灵敏的仪器来监控活体动物体内的细胞活动、蛋白表达情况和基因行为。近年来，可见光成像在生物医学的各个方面得到了广泛的应用。随着成像技术和检测仪器的不断发展，现已从平面二维成像逐渐发展为立体三维成像。 三维成像技术在靶点的空间定位、与器官的关系，及绝对定量方面都有了很大的进展。 本文就三维成像技术的原理、应用和发展前景进行了简要的综述。
关键词：体内光学成像；三维活体成像；生物发光；荧光；肿瘤转移
中图分类号：R445.1; Q631.11　　文献标识码：A

Current developments of 3-D optical in vivo imaging technology in animal

YANG Hua-yu1, HAN Yu2, Dong Hong-ying2, ZHAO Chun-lin2*

(1Department of Liver Surgery, PUMCH, CAMS, Beijing 100730, China;2Longmed, Inc/CS-Biotech, Inc., Beijing 100037, China)

Abstract: Abstract: Bioluminescence and fluorescence have been proved to be a versatile and useful tool for animal in vivo imaging. Optical imaging employs sensitive instrument to monitor the activities of cell, protein and gene in vivo. It has been used widely in biomedical research and drug discoveries.  With the recent development of the technology and instruments, the 2Dimentional imaging has developed to 3Dimential imaging in vivo. 3-D imaging will provide researchers not only detailed spatial information of the targets, its relationship with animal organs, but more quantitative results in vivo. This paper reviews of the current principles, developments and applications of the 3-D optical in vivo imaging technologies.
Key words: animal imaging; in vivo optical imaging; 3-Dimentional bioluminescence; 3-Dimentional fluorescence;  tumor metastasis