《生命科学》 2008, 20(1):

G蛋白偶联受体激活的单分子研究进展

刘　飞，张幼怡*

(北京大学第三医院血管医学研究所 教育部分子心血管学重点实验室，北京 100083)

摘　要：摘　要：G蛋白偶联受体是体内最大的受体超家族，它们参与调节生物体内多种生理功能与病理过程。G蛋白偶联受体的分子内构象变化与G蛋白的偶联以及受体的二聚化等是G蛋白偶联受体激活的重要基本过程。借助于单分子研究手段，在G蛋白偶联受体激活方面取得了重要进展。本文将就这些方面进行简要的综述。
关键词：G蛋白偶联受体；单分子；G蛋白；构象；二聚化
中图分类号：Q-33；Q503　　文献标识码：A

Progress of single-molecule detection of G protein-coupled receptor activation

LIU Fei, ZHANG You-yi*

(Institute of Vascular Medicine, Peking University Third Hospital, and Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Beijing 100083, China)

Abstract: Abstract: G protein-coupled receptors (GPCRs) which constitute the largest superfamily of receptors in human genome, regulate a variety of physiological functions and pathological processes. The changes of intramolecular conformation of GPCRs, their coupling to G-protein and receptor dimerization represent the basic respects in GPCRs activation. Benefited from the improvement of single-molecule detection techniques, significant progresses are achieved in the studies of this field, which will be briefly reviewed here.
Key words: GPCRs; single-molecule; G-protein; conformation; dimerization