《生命科学》 2007, 19(4):

一种利用植物病毒载体表达外源蛋白的新平台-Magnifection

杨正安^1,3，丁玉梅²，张应华¹，刘飞虎^3*

（1云南农业大学园林园艺学院，昆明650201；2云南省农业生物技术重点实验室，昆明650223；3云南大学生命科学学院，昆明650091）

摘　要：摘　要：本文介绍国内外在利用植物病毒表达载体生产药物蛋白的研究现状，并对这一领域取得的最新突破进行重点阐述，包括Magnifection的原理、技术流程及利用其生产重组药用蛋白的优势、存在的问题等。最后，结合相关经验介绍利用植物病毒表达载体生产药物蛋白的应用前景及对该技术改进的建议。
关键词：Magnifection；植物；病毒载体；瞬时表达
中图分类号：S432.41　　文献标识码：A

Magnifection: a new platform for expression of foreign protein using a plant viral朾ased vector

YANG Zheng{$39}an^1,3, DING Yumei², ZHANG Yinghua¹, LIU Feihu^3*

(1 College of Horticulture and Landscape, Yunnan Agricultural University, Kunming 650201, China; 2 Key Lab of Agricultural Biotechnology of Yunnan, Kunming 650223, China; 3 School of Life Sciences, Yunnan University,Kunming 650091, China)

Abstract: Abstract: This article reviews the current research status of expression of recombinant proteins using plant viral-based vector, and more attentions are focused on the principle and the operation procedure of Magnifection and its advantages and limitation for the production of recombinant pharmaceutical proteins，lastly, we introduce its application in diverse aspects of understanding the mechanisms of RNA processing and nuclear export in plant, gene replacement, gene insertion, epitope presentation, gene complementation and commercial purpose. Our experiences in modification of the Magnifection technique are also provided.
Key words: Magnifection；plants；viral vector；transient expression