《生命科学》 2025, 37(3): 321-328

面向体外诊断的体液蛋白质组学分析前因素研究进展

李　玙^1,2,3 , 应　超^1,2,3 , 李奇萌⁴ , 赵立芳^4,* , 蔡燕宁^1,2,3,4,*

¹首都医科大学宣武医院神经生物学研究室，北京 100053 ²教育部神经变性病重点实验室，北京 100 ³国家老年疾病临床医学研究中心，北京 100053 ⁴首都医科大学宣武医院临床样本中心，北京 100053

摘　要：

在体外诊断领域，蛋白质组学研究对于生物标志物的发现、验证和确认至关重要。越来越多证据表明，不同的样本处理和操作步骤会影响蛋白质的丰度和检测的可靠性。体外诊断中的大多数异常和错误都发生在样本的分析前阶段，这些变量会对分析物的测定结果产生巨大影响。然而，目前关于分析前变量对样品采集和储存后蛋白质的稳定性以及完整性的影响尚不完全清楚。本文系统综述了分析前变量对生物体液蛋白质组学研究的影响，以期为蛋白质组学研究提供新的思路。

通讯作者：赵立芳 , Email:lfzhao2021@163.com 蔡燕宁 , Email:yanningcaimailbox@163.com

Advances in the analysis of pre-analytical factors for in vitro diagnostic-oriented proteomics of body fluids

LI Yu^1,2,3 , YING Chao^1,2,3 , LI Qi-Meng⁴ , ZHAO Li-Fang^4,* , CAI Yan-Ning^1,2,3,4,*

¹Neurobiology Laboratory, Xuanwu Hospital, Capital Medical University, Beijing 100053, China ²Key Laboratory of Neurodegenerative Diseases, Ministry of Education, Beijing 100053, China ³National Clinical Research Center for Geriatric Diseases, Beijing 100053, China ⁴Clinical Sample Center, Xuanwu Hospital, Capital Medical University, Beijing 100053, China

Abstract:

In the domain of in vitro diagnostics, proteomics research plays a crucial role in the discovery, validation, and confirmation of biomarkers. Increasing evidence suggests that various sample handling and manipulation procedures significantly influence protein abundance and the reliability of assays. The majority of abnormalities and errors in in vitro diagnostics are observed during the pre-analytical phase of sample processing, and these variables can significantly influence the outcomes of the analyte assay. Nevertheless, the influence of pre-analytical variables on the stability and integrity of proteins following sample collection and storage remains inadequately comprehended. In this article, we systematically reviewed and analyzed the impact of pre-analytical variables on proteomics research of body fluids, aiming to provide new insights for proteomics research.

Communication Author：ZHAO Li-Fang , Email:lfzhao2021@163.com CAI Yan-Ning , Email:yanningcaimailbox@163.com