《生命科学》 2024, 36(11): 1330-1338

DNA甲基化研究的分析前影响因素

胡松年^1,2,3 , 丁　晖^1,2,3 , 赵立芳^4,* , 蔡彦宁^1,2,3,4,*

¹首都医科大学宣武医院神经生物学研究室，北京 100053 ²教育部神经变性病重点实验室，北京 100053 ³国家老年疾病临床医学研究中心，北京 100053 ⁴首都医科大学宣武医院临床样本中心，北京 100053

摘　要：

DNA 甲基化是一种导致基因表达沉默的动态表观遗传修饰，在临床疾病生物标志物研究中受到了广泛关注。然而，由于缺乏标准化的分析前处理方式，当前DNA 甲基化分析结果的准确性和可靠性无法保证。本文旨在探讨不同分析前因素对DNA 甲基化分析结果的影响，并对部分因素给出适当的处理建议，以期为DNA 甲基化相关临床研究提供新见解。

通讯作者：赵立芳 , Email:lfzhao2021@163.com 蔡彦宁 , Email:yanningcaimailbox@163.com

Pre-analytical influencing factors in DNA methylation study

HU Song-Nian^1,2,3 , DING Hui^1,2,3 , ZHAO Li-Fang^4,* , Cai Yan-Ning^1,2,3,4,*

¹Neurobiology Laboratory, Xuanwu Hospital, Capital Medical University, Beijing 100053 ²Key Laboratory of Neurodegenerative Diseases, Ministry of Education, Beijing 100053, China ³National Clinical Research Center for Geriatric Diseases, Beijing 100053, China ⁴Clinical Sample Center, Xuanwu Hospital, Capital Medical University, Beijing 100053, China

Abstract:

DNA methylation is a dynamic epigenetic modification that leads to silencing of gene expression and has received extensive attention in clinical biomarker study. However, the accuracy and reliability of current DNA methylation analysis are uncertain due to lacking of standardized pre-analysis procedures. The purpose of this paper is to explore the influence of different factors on DNA methylation analysis and to give appropriate treatment suggestions for some factors to provide new insights for clinical research on DNA methylation.

Communication Author：ZHAO Li-Fang , Email:lfzhao2021@163.com Cai Yan-Ning , Email:yanningcaimailbox@163.com