《生命科学》 2024, 36(7): 943-950

人诱导多能干细胞来源的小胶质样细胞模型优化及其在阿尔茨海默症研究中的应用

李　颖 , 魏子巍 , 钱　欣 , 丁红群 , 刘思雨 , 赵君瑜 , 张维宁^*

江苏大学医学院，镇江 212013

摘　要：

在过去的二十年中，人诱导多能干细胞在体外研究人类神经发育机制、疾病建模和药物发现等领域应用广泛，特别是在神经退行性疾病领域。相比于其他研究模型，以诱导多能干细胞衍生的小胶质细胞为基础的体外模型更有利于研究阿尔茨海默症背后的病理机制。然而，不同来源的诱导多功能干细胞在分化潜能、表观遗传状态等方面差异较大，其体外分化能力易受重编程方法和培养体系影响。因此，本文综述了近年来人诱导多能干细胞来源的小胶质样细胞模型的发展现状及其在阿尔茨海默症研究中的应用，希望能为今后小胶质细胞的研究提供有效参考。

通讯作者：张维宁 , Email:wnzhang@ujs.edu.cn

Optimization of human induced pluripotent stem cell-derived microglia-like cell model and its application in the study of Alzheimer's disease

LI Ying , WEI Zi-Wei , QIAN Xin , DING Hong-Qun , LIU Si-Yu , ZHAO Jun-Yu , ZHANG Wei-Ning^*

School of Medicine, Jiangsu University, Zhenjiang 212013, China

Abstract:

Over the past two decades, human induced pluripotent stem cells have been widely used for in vitro studies of human neurodevelopmental mechanisms, disease modeling, and drug discovery, especially in the field of  neurodegenerative diseases. Compared to other research models, in vitro models based on induced pluripotent stem cell-induced microglia-like cells are more favorable for studying the pathological mechanisms behind Alzheimer's disease. However, induced multifunctional stem cells from different sources vary widely in differentiation potential and epigenetic status, and their in vitro differentiation ability is susceptible to reprogramming methods and culture systems. Therefore, this paper reviews the current development of microglia-like cell models derived from human induced pluripotent stem cells and their applications in Alzheimer's disease research in recent years, hoping to provide an effective reference for future microglia research.

Communication Author：ZHANG Wei-Ning , Email:wnzhang@ujs.edu.cn