《生命科学》 2023, 35(11): 1434-1441

男性精浆代谢组学的研究现况与进展

吴雅茹 , 张　洁^*

厦门大学公共卫生学院，分子疫苗学与分子诊断学国家重点实验室，厦门 361102

摘　要：

男性精子质量下降是全球范围内的重大公共卫生问题。然而，相关疾病的精准诊断十分困难，致病机制尚未完全阐明。精浆是男性生殖领域研究的理想样本，精浆代谢组学在探索男性生殖领域生物标志物及分子机理方面具有广阔的应用前景。本文综述了2011 年至2022 年典型的精浆代谢组学研究，介绍了核磁共振、气相色谱- 质谱联用、液相色谱- 质谱联用和拉曼光谱的技术特点及应用现状。同时，本综述从精浆代谢组学方法、精浆代谢标志物及其涉及的代谢通路等多角度出发，总结男性生殖系统疾病和环境暴露等因素导致的精浆代谢改变，以及主要涉及的通路，包括能量代谢、脂质代谢、氨基酸代谢和类固醇代谢等。最后，分析了精浆代谢组的研究难点，并展望了其未来的发展趋势。

通讯作者：张　洁 , Email:jie.zhang@xmu.edu.cn

Current status and progress of male seminal plasma metabolomics research

WU Ya-Ru , ZHANG Jie^*

State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, China

Abstract:

Male sperm quality decline is a major public health problem worldwide. However, accurate diagnosis of the associated diseases is still challenging, and the pathogenesis remains unclear. Seminal plasma is an ideal sample for male reproduction research, and  seminal plasma metabolomics has  promising applications in investigating potential biomarkers and molecular  mechanisms of male reproduction. In this article, we review typical semen metabolomics studies from 2011 to  2022, and introduce the technical  characteristics and application status of nuclear magnetic resonance, gas  chromatography-mass spectrometry, liquid chromatography-mass  spectrometry, and Raman spectroscopy. Additionally, we summarize the  alterations in seminal plasma  metabolism attributed to male reproductive disorders and  environmental exposures from multiple perspectives, including seminal plasma metabolomic methods, seminal plasma metabolic markers and their involved  metabolic pathways, which mainly include energy metabolism, lipid  metabolism, amino acid metabolism and steroid metabolism. Finally, we analyze the research difficulties of semen metabolomics in the field of male reproduction and propose future development trends.

Communication Author：ZHANG Jie , Email:jie.zhang@xmu.edu.cn