《生命科学》 2023, 35(8): 994-1003

类器官培养方法及家畜类器官研究进展

张　言 , 朱春玲 , 杨　蕊 , 张博洋 , 陈斓歆 , 赵彦森 , 王玥琦 , 唐　博^* , 张学明^*

吉林大学动物医学学院，动物胚胎工程吉林省重点实验室，长春 130062

摘　要：

类器官(organoid) 是由干细胞在体外培育而成的一种三维(3D) 细胞培养物，其中包含多种细胞类型的自组装。类器官是生物医学领域内近年来的热门前沿技术之一，可用于发育、内稳态、再生、疾病建模和药物研发等领域的相关研究。不同类器官的培养方法存在差异，因此了解相关进展对成功构建合适的类器官模型非常重要。家畜与人类生活息息相关，通过构建类器官的方式以其为研究对象，对人类的饮食安全、医疗保健、精神健康都具有重要意义。本文评述了类器官的培养方式及家畜类器官研究方面的进展，以便为后期相关研究提供参考。

通讯作者：唐　博 , Email:tang_bo@jlu.edu.cn 张学明 , Email:zhangxuem@jlu.edu.cn

Advances in organoid methodology and livestock organoids

ZHANG Yan , ZHU Chun-Ling , YANG Rui , ZHANG Bo-Yang , CHEN Lan-Xin , ZHAO Yan-Sen , WANG Yue-Qi , TANG Bo^* , ZHANG Xue-Ming^*

Key Laboratory of Animal Embryo Engineering of Jilin Province, College of Veterinary Medicine, Jilin University, Changchun 130062, China

Abstract:

Organoid is a three-dimensional (3D) cell culture created from stem cells grown in vitro, which contains self-assembly of multiple cell types. It’s one of the recent hot frontier technology for studying the development, homeostasis, regeneration, disease modeling, and drug discovery, etc. Since the culture methods of different organoids are different, it is important to understand the relevant progress for construction of suitable organoid models. Domestic animals are closely related to human life, it is of great importance to study them by constructing organoids for human diet safety, health care, and mental health. Here we review the culture methods of multiple organoids and the progress of livestock organoids, aiming to provide references for the future research.

Communication Author：TANG Bo , Email:tang_bo@jlu.edu.cn ZHANG Xue-Ming , Email:zhangxuem@jlu.edu.cn