《生命科学》 2023, 35(3): 315-328

基于表面修饰的活体细菌药物及递送策略

张一帆^1,2 , 林思思^2,* , 吴　冯² , 刘尽尧^2,*

¹上海师范大学化学与材料科学学院，上海 200234 ²上海交通大学医学院，仁济医院，上海市肿瘤研究所癌 基因及相关基因国家重点实验室，分子医学研究院，上海市核酸化学与纳米医学重点实验室，上海 200127

摘　要：

活体细菌在多种疾病防治领域显示出巨大的应用前景。然而，以细菌为基础的“活体材料”在生物医药应用中普遍面临生物利用度低、作用缺乏靶向性、治疗效果不佳和安全性等问题。表面工程技术为克服上述问题，发展新型活体细菌药物及其先进递送平台提供了重要的手段。该文首先介绍了活体细菌表面修饰的物理和化学手段；进一步讨论了表面修饰后的活体细菌药物所具有的不良环境耐受性、特定作用位点靶向性和定向运动等优良特性；最后总结了表面修饰改造的活体细菌在肿瘤和肠炎等不同疾病的诊断、成像和治疗中的应用。这项工作将有助于推进细菌疗法和活体细菌药物的进一步发展与应用。

通讯作者：林思思 , Email:SisiLinjy@outlook.com 刘尽尧 , Email:jyliu@sjtu.edu.cn

Fabrication and delivery of live bacterial therapeutics by surface modification

ZHANG Yi-Fan^1,2 , LIN Si-Si^2,* , WU Feng² , LIU Jin-Yao^2,*

¹College of Chemistry and Materials Science, Shanghai Normal University, Shanghai 200234, China ²State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Institute of Molecular Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China

Abstract:

Live bacterial therapeutics have shown great potential in treating various diseases. However, conventional live bacterial agents always suffer from low bioavailability, lack of targeting ability, poor therapeutic effects, and low safety in different biomedical applications. Surface modification provides a promising tool to overcome these challenges, which promotes the development of live bacterial therapeutics and their advanced delivery systems. In this review, we first introduce the physical and chemical surface modification means of live bacteria. Then, the superior features of surface-modified live bacteria, including the tolerance for adverse environmental conditions, site-specific targeting ability, and directional movement, are discussed. Lastly, we summarize the biomedical applications of surface-modified live bacteria in the diagnosis, imaging, and treatment of different diseases. This work will inspire the development and application of next-generation live bacterial therapeutics.

Communication Author：LIN Si-Si , Email:SisiLinjy@outlook.com LIU Jin-Yao , Email:jyliu@sjtu.edu.cn