《生命科学》 2023, 35(3): 252-266

减毒沙门氏菌介导的肿瘤基因治疗

李晨阳¹ , 李　强¹ , 华子春^1,2,3,*

¹南京大学生命科学学院，医药生物技术国家重点实验室，南京 210023 ²南京吉芮康生物科技研究院有限 公司，南京 210044 ³常州南京大学高新技术研究院，江苏靶标生物医药研究所有限公司，常州 213164

摘　要：

细菌介导的肿瘤治疗最早可追溯到一个多世纪前，其具有的瘤内定植增殖、靶向性高的特性，是放化疗、免疫检查点调控等传统肿瘤治疗方法难以企及的。近年来，随着合成生物学的发展，该疗法得到了极大的发展，以沙门氏菌为代表的多种抗肿瘤菌株相继涌现。本文综述了基于平衡细菌抗肿瘤疗效与毒副作用改造策略的沙门氏菌减毒菌株构建，以及减毒菌株作为药物递送载体介导肿瘤基因治疗的现状和进展。

通讯作者：华子春 , Email:zchua@nju.edu.cn

Gene therapy mediated by attenuated Salmonella

LI Chen-Yang¹ , LI Qiang¹ , HUA Zi-Chun^1,2,3,*

¹State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing Univercity, Nanjing 210023, China ²Nanjing Generecom Biotechnology Co., Ltd., Nanjing 210044, China ³Changzhou High-Tech Research Institute of Nanjing University and Jiangsu Target Pharma Laboratories Inc., Changzhou 213164, China

Abstract:

Bacteria-mediated tumor therapy can be traced back to more than one century ago. Its characteristic of intratumoral colonization and proliferation with high targeting capacity is unachievable by traditional tumor therapies, such as radiotherapy and immune checkpoint modulation. In recent years, with the development of synthetic biology, this therapy has been greatly developed, and a variety of antitumor strains represented by Salmonella have been found. This paper reviews the construction of attenuated Salmonella strains based on a modification strategy that balances bacterial antitumor efficacy and virulence, as well as the current status and development of attenuated strains as drug delivery vehicles mediating oncogene therapy.

Communication Author：HUA Zi-Chun , Email:zchua@nju.edu.cn