《生命科学》 2022, 34(10): 1227-1239

类精子干细胞介导的遗传改造

杨振华¹ , 李劲松^1,2,3,4,*

¹上海科技大学生命科学与技术学院，上海 201210 ²中国科学院分子细胞科学卓越创新中心/上海生物化学与细胞生物学研究所，上海市分子男科学重点实验室，细胞生物学国家重点实验室，上海 200031 ³中国科学院大学，北京 100049 ⁴中国科学院大学杭州高等研究院生命科学学院，杭州 310024

摘　要：

在基因编辑领域，以CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9) 为代表的技术的广泛应用，使得科学家能快速、高效、精确地对靶基因序列进行遗传改造。但是，由于包括人类在内的真核细胞二倍性的特点，要获得纯合编辑的细胞系和模式动物仍需大量的时间和人力成本。类精子干细胞(DKO-AG-haESCs) 系统的建立及其与CRISPR-Cas9 技术的结合则可以快速地在基因、染色体等水平上进行任意遗传操作并转化为动物个体，实现疾病模拟。本文将对类精子干细胞介导的遗传改造进行总结和讨论。

 

通讯作者：李劲松 , Email:jsli@sibcb.ac.cn

Sperm-like stem cell-mediated genome editing

YANG Zhen-Hua¹ , LI Jin-Song^1,2,3,4,*

¹School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China ²State Key Laboratory of Cell Biology, Shanghai Key Laboratory of Molecular Andrology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai 200031, China ³University of Chinese Academy of Sciences, Beijing 100049, China ⁴School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, China

Abstract:

The extensive application of technology represented by CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9), make the genetic modification more efficient. However, it remains a laborious endeavor to obtain homozygous gene-edited cell lines and model animals because of the diploidy of human cells and model organisms. The establishment of sperm-like stem cells (DKO-AG-haESCs) and its combination with CRISPR-Cas9 technology can rapidly transform genetic operation at the gene level and chromosome level into animals so that we can realize disease simulation. Here, we will summarize the sperm-like stem cells mediated gene editing.

Communication Author：LI Jin-Song , Email:jsli@sibcb.ac.cn