《生命科学》 2022, 34(7): 871-879

启动子改造优化策略在微生物代谢工程中的应用

许　莹¹ , 唐丽娟¹ , 张部昌² , 刘　静^1,*

¹安徽农业大学生命科学学院，合肥 230036 ²安徽大学物质科学与信息技术研究院，合肥 230601

摘　要：

启动子是基因表达必需的顺式作用元件，在基因转录水平调控中居于至关重要的地位，启动子活性的强弱直接影响基因表达的水平。在微生物代谢工程途径改造过程中，常见的策略是利用不同强度的启动子对目的基因表达进行精细的调控，实现各中间代谢产物在生成与利用之间的协调平衡，提高代谢产物的合成效率。本文首先简述了启动子的特征与功能，以及内源性启动子在应用上的局限性，提出了启动子改造及优化的必要性和意义，重点概述了目前启动子工程领域对启动子进行修饰改造的策略以及获得的研究进展，并对启动子改造优化在代谢工程和合成生物学领域的应用前景进行了展望。

 

通讯作者：刘　静 , Email:liujing9261@ahau.edu.cn

Application of promoter optimization strategies in microbial metabolic engineering

XU Ying¹ , TANG Li-Juan¹ , ZHANG Bu-Chang² , LIU Jing^1,*

¹School of Life Sciences, Anhui Agricultural University, Hefei 230036, China ²Institution of Physical Science and Information Technology, Anhui University, Hefei 230601, China

Abstract:

Promoter is a cis-acting element essential for gene expression, and is crucial in the regulation of gene transcription. The strength of promoter activity directly determines the level of gene expression. In microbial metabolic engineering, a common strategy is to use the promoters with different strengths to precisely regulate the expression of target genes, achieving the coordinated balance between the generation and utilization of the intermediate metabolites, and improving the synthesis efficiency of the metabolites. This article briefly introduces the features and functions of promoters, proposes the necessity and significance of promoter modification and optimization, and detailly reviews the strategies and the applications of promoter optimization in metabolic engineering and synthetic biology.

 

Communication Author：LIU Jing , Email:liujing9261@ahau.edu.cn