《生命科学》 2022, 34(6): 613-623

驱动蛋白MCAK的微管解聚机制研究进展

彭　川 , 耿轶钊^*

河北工业大学理学院，生物物理研究所，天津 300401

摘　要：

    有丝分裂着丝粒相关驱动蛋白(MCAK) 是驱动蛋白-13 家族的代表性成员，在细胞分裂的多个过程中起作用。与传统的驱动蛋白不同，MCAK 蛋白的生物学功能不是通过沿微管轨道的连续行走实现物质输运，而是从微管末端解聚微管蛋白，从而实现对微管动力学的调控。因此，MCAK 蛋白是一种微管解聚蛋白，其沿微管轨道的运动过程以及解聚微管蛋白时的力学- 化学循环过程都与其他驱动蛋白家族有很大的差异。该文对MCAK 蛋白微管解聚过程的研究进展进行综述，尤其对高分辨率分子结构展现的MCAK蛋白在解聚微管末端蛋白时的1:2 复合物模型进行了详细讨论。

通讯作者：耿轶钊 , Email:gengyz@hebut.edu.cn

Research progress of mitotic centromere-associated kinesin’s microtubule-depolymerization mechanism

PENG Chuan , GENG Yi-Zhao^*

Institute of Biophysics, School of Science, Hebei University of Techonology, Tianjin 300401, China

Abstract:

Mitotic centromere-associated kinesin (MCAK) is the representative member of the kinesin-13 family and plays multiple roles in cell division. Different from conventional kinesins, the biological function of MCAK is not cargo transportation which is realized through processive movement along microtubule lattice. MCAK can regulate the microtubule dynamics through depolymerizing the microtubule from two ends. Therefore, MCAK is a kind of microtubule regulators. Its walking and mechanochemical processes are different from other kinesin families. In this paper, we reviewed the research progress of the microtubule-depolymerization mechanism of MCAK, especially the 1:2 complex model revealed by the high-resolution crystal and cryo-electron microscope structures.

Communication Author：GENG Yi-Zhao , Email:gengyz@hebut.edu.cn