《生命科学》 2022, 34(1): 91-98

类器官技术在泌尿系统疾病研究中的应用

孟　倩^1,2 , 冯晓莹^1,2 , 余　磊³ , 倪文秀² , 陈　巍^1,* , 黄卫人^1,*

¹深圳市第二人民医院，医学合成生物学临床应用关键技术国家地方联合工程实验室，深圳 518036 ²汕头大学医学院，汕头 515041 ³中国科学院深圳先进技术研究院，深圳合成生物学创新研究院，深圳 518055

摘　要：

类器官是由干细胞、正常或肿瘤细胞在体外培养获得的，与人体组织结构和功能相似的一种3D细胞模型。近年来，随着类器官培养技术的不断发展和完善，泌尿系统相关类器官模型纷纷建立。该文概述了类器官培养技术在泌尿系统相关研究领域中的应用，包括器官发育及组织修复、肾毒性测试、泌尿系统疾病模型的构建、个性化抗肿瘤药物筛选，同时讨论了泌尿系统类器官模型应用的局限性，并对该项技术广阔的应用前景进行展望。

通讯作者：陈　巍 , Email:jessie_chenwei@163.com 黄卫人 , Email:pony8980@163.com

Application of organoid technology in the research of urological system diseases

MENG Qian^1,2 , FENG Xiao-Ying^1,2 , YU Lei³ , NI Wen-Xiu² , CHEN Wei^1,* , HUANG Wei-Ren^1,*

¹State and Local Government Joint Engineering Laboratory of Synthetic Biology Medicine and Clinical Application of Key Technologies, Shenzhen Second Hospital, Shenzhen 518036, China ²Medical College, Shantou University, Shantou 515041, China ³Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China

Abstract:

Organoid is a cellular three-dimensional in vitro model derived from stem cells, normal or tumor cells, and is capable of recapitulating the structure and function of native organs. Over the past decade, this rapidly evolving field has seen the establishment of various organoid models of urinary diseases. This review summarizes the application of organoid culture technology in the research field of urinary system, including organ development and tissue repair, drug nephrotoxicity testing, construction of urinary system disease models, and personalized anti-tumor drug screening. The limitations and promising application prospects of urological organoid models are also discussed.

Communication Author：CHEN Wei , Email:jessie_chenwei@163.com HUANG Wei-Ren , Email:pony8980@163.com