《生命科学》 2022, 34(1): 83-90

纳米抗体在肿瘤分子影像诊断中的研究进展

陈　曲¹ , 杨晓梅² , 施　维² , 谢深霞^3,* , 江梦捷⁴ , 孙树阳¹ , 卢小玲^1,*

¹广西医科大学口腔医学院，广西纳米抗体研究重点实验室，广西纳米抗体国际联合研究中心，南宁 530021 ²广西医科大学基础医学院，广西纳米抗体研究重点实验室，广西纳米抗体国际联合研究中心，南宁 530021 ³广西医科大学药学院药学实验中心，广西纳米抗体研究重点实验室，广西纳米抗体国际联合研究中心，南宁 530021 ⁴广西医科大学附属肿瘤医院，南宁 530021

摘　要：

肿瘤的分子影像诊断是以肿瘤关键分子作为识别靶点，借助特异性分子探针和高灵敏成像设备，在活体状态下对肿瘤进行成像的技术。在临床实践中因其具备可定性、定量、可视化的优点，近年来已经成为肿瘤早期诊断、肿瘤术前治疗的重要依据，在疗效监测中起到不可或缺的作用。作为可结合肿瘤特异性靶标的分子探针的重要载体，纳米抗体(nanobody, Nb) 因具备体积小、稳定性强、亲和力高、免疫原性弱等显著优势，在肿瘤分子影像诊断领域中的作用日渐突出。该文就纳米抗体的结构特征、基于纳米抗体的分子探针及其在分子影像应用领域的最新进展进行综述，同时也讨论了纳米抗体应用于肿瘤成像的临床局限性，并对其未来的研究应用进行了展望。

通讯作者：谢深霞 , Email:xieshenxia@gxmu.edu.com 卢小玲 , Email:luwuliu@163.com

Progress of molecular imaging of nanobody in the diagnosis of cancers

CHEN Qu¹ , YANG Xiao-Mei² , SHI Wei² , XIE Shen-Xia^3,* , JIANG Meng-Jie⁴ , SUN Shu-Yang¹ , LU Xiao-Ling^1,*

¹International Nanobody Research Center of Guangxi, Key Laboratory for Nanobody Research of Guangxi, Stomatology College of Guangxi Medical University, Nanning 530021, China ²International Nanobody Research Center of Guangxi, Key Laboratory for Nanobody Research of Guangxi, Basic Medical College of Guangxi Medical University, Nanning 530021, China ³International Nanobody Research Center of Guangxi, Key Laboratory for Nanobody Research of Guangxi, Pharmacy Experimental Center, Pharmacy College of Guangxi Medical University, Nanning 530021, China ⁴Tumor Hospital of Guangxi Medical University, Nanning 530021, China

Abstract:

Tumor molecular imaging diagnosis is a technology that uses specific molecular probes and highly sensitive imaging equipment to image tumors in a living status, through recognizing key tumor targets. In clinical practice, tumor molecular imaging provides important evidence for early diagnosis and preoperative treatment of tumors, and plays an indispensable role in treatment effectiveness monitoring in recent years, because of its qualitative, quantitative and visual advantages. As an important carrier of molecular probes that can bind to tumorspecific targets, nanobody (Nb) has greatest strengths in the field of tumor molecular imaging diagnosis due to its small size, strong stability, high affinity, and weak immunogenicity. Therefore, Nb plays an increasingly prominent role in the field of tumor molecular imaging diagnosis. This article reviews the structural characteristics of Nb, as well as the Nb-based molecular probes and their most recent advances in molecular imaging applications. Meanwhile, this article also discusses the clinical limitations of Nb for tumor imaging, and prospects some future research applications.

Communication Author：XIE Shen-Xia , Email:xieshenxia@gxmu.edu.com LU Xiao-Ling , Email:luwuliu@163.com