《生命科学》 2021, 33(12): 1476-1482
代谢研究新技术助力合成生物学
摘 要:
合成生物学通过构建人工代谢途径,不仅可以发展高效的生物制造技术,还可以用于人类重要疾病的诊疗,为催生新的生物产业革命、促进经济可持续发展提供了重大机遇。该文围绕代谢组与代谢流量分析以及代谢建模、单细胞代谢表型组分析与分选、代谢物生物传感与时空调控等前沿技术,介绍现有状况和水平,综述分析这些技术如何助推合成生物学设计- 构建- 测试- 学习(DBTL) 循环,并对其发展前景进行展望。
通讯作者:杨 琛 , Email:cyang@cemps.ac.cn 徐 健 , Email:xujian@qibebt.ac.cn 杨 弋 , Email:yiyang@ecust.edu.cn
Abstract:
Cellular metabolism is being engineered through synthetic biology for an increasingly diverse array of applications, from chemical production to human health. In this review, we highlight the recent advances in the techniques of metabolic sciences, including metabolomics, metabolic flux analysis, genome-scale metabolic modeling, single-cell metabolic phenome, Raman-activated cell sorting, spatiotemporal metabolic analysis, single-cell optogenetic control, and metabolic phenomics. These techniques are accelerating the design, build, test, and learn (DBTL) cycles for synthetic biology. The emerging challenges and future developments in these techniques and their applications in synthetic biology are discussed.
Communication Author:YANG Chen , Email:cyang@cemps.ac.cn XU Jian , Email:xujian@qibebt.ac.cn YANG Yi , Email:yiyang@ecust.edu.cn