《生命科学》 2021, 33(12): 1462-1468

基因组编辑技术及未来发展

赵东东¹ , 宗　媛² , 尹　蕾³ , 毕昌昊^1,* , 王　金^4,* , 高彩霞^2,* , 张学礼^1,*

¹中国科学院天津工业生物技术研究所，天津 300308 ²中国科学院遗传与发育生物学研究所，北京 100101 ³上海师范大学，上海 200234 ⁴深圳市第二人民医院转化医学研究院，深圳 518000

摘　要：

基因组编辑技术是合成生物学的一项核心使能技术。CRISPR基因组编辑技术在生命科学领域掀起了一场全新的技术革命，助推了合成生物学快速发展。然而，目前CRISPR基因组编辑技术的性能尚有欠缺，智能设计、表达和投递系统等相关技术也不能满足医疗和农业领域的应用需求。未来基因组编辑技术的发展方向，一方面亟待开发更精准、高效、全面和智能的CRISPR基因组编辑技术；另一方面，需利用大数据分析和人工智能技术，开发CRISPR之外全新的颠覆性基因组编辑技术。

通讯作者：毕昌昊 , Email:bi_ch@tib.cas.cn 王　金 , Email:wangj01@hotmail.com 高彩霞 , Email:cxgao@genetics.ac.cn 张学礼 , Email:zhang_ xl@tib.cas.cn

Genome editing technology and future development

ZHAO Dong-Dong¹ , ZONG Yuan² , YIN Lei³ , BI Chang-Hao^1,* , WANG Jin^4,* , GAO Cai-Xia^2,* , ZHANG Xue-Li^1,*

¹Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China ²Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China ³Shanghai Normal University, Shanghai 200234, China ⁴Institute of Translational Medicine, Shenzhen Second People's Hospital, Shenzhen 518000, China

Abstract:

Genome editing is an incredibly enabling synthetic biology technology. CRISPR genome editing technology represents a technological revolution across all life sciences. However, currently CRISPR genome editing technologies are still limited by many challenges, and other related technologies such as intelligent design, expression and delivery systems are unable to meet requirements of applying CRISPR editing across medicine and agriculture. For future genome editing technology developments, it is necessary to generate more accurate, efficient, comprehensive and intelligent CRISPR genome editing technologies. Additionally, big data analyses and artificial intelligence approaches are essential towards developing novel subversive genome editing technologies that differ from CRISPR approaches.

Communication Author：BI Chang-Hao , Email:bi_ch@tib.cas.cn WANG Jin , Email:wangj01@hotmail.com GAO Cai-Xia , Email:cxgao@genetics.ac.cn ZHANG Xue-Li , Email:zhang_ xl@tib.cas.cn