《生命科学》 2021, 33(12): 1452-1461

细胞工厂底盘抗逆属性的分子调控进展

刘　夏¹ , 秦　磊² , 李　春^1,2,*

¹北京理工大学化学与化工学院，生物化工研究所，医药分子科学与制剂工程工信部重点实验室， 北京 100081 ²清华大学化学工程系，工业生物催化教育部重点实验室，北京 100084

摘　要：

以微生物作为底盘细胞的绿色生物制造是化工制造领域的重要发展方向，是实现“双碳计划”和绿色经济的重要方式。微生物细胞工厂在工业应用中容易面临各种环境胁迫，影响细胞生长和产物合成，而维持最佳生产条件所需的高能耗和高成本制约了绿色生物制造的进一步发展。通过合成生物学手段改造底盘菌株，提高底盘菌株抗逆性是生物制造节能减排的有效途径。该文介绍了细胞工厂抗逆特性及其在生物制造中的产业化应用，并且综述了底盘细胞抗性的分子调控进展及改造策略。细胞工厂底盘抗逆属性的提升将推动绿色生物制造的发展，助力“碳中和、碳达峰”目标的实现。

通讯作者：李　春 , Email:lichun@tsinghua.edu.cn

Progress on molecular regulation of stress resistance properties for cell factory

LIU Xia¹ , QIN Lei² , LI Chun^1,2,*

¹Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Ministry of Industry and Information Technology, Institute of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China ²Key Laboratory for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China

Abstract:

Green biomanufacturing based on microbes as chassis cells is an important trend for the development of chemical engineering manufacturing, and is an important approach to realize the "dual-carbon plan" and green economy. Industrial applications of cell factories often face various environmental stresses that affect cell growth and production. The high energy consumption and high costs required for optimal production conditions restrict the further development of the green biomanufacturing. Engineering chassis strains through synthetic biology technologies is the effective way to improve the robustness of strains. This paper introduces the resistance properties and the industrial application of cell factory. The progress of molecular regulation in chassis cell resistance and modification strategies are summarized in depth. The improvement of stress resistance properties of cell factory chassis will promote the development of green biomanufacturing and help to achieve the goal of "carbon peak and neutrality".

Communication Author：LI Chun , Email:lichun@tsinghua.edu.cn