《生命科学》 2021, 33(3): 326-336

RNA化学标记技术

方振天 , 卢子昂 , 方　鑫 , 翁小成^* , 周　翔^*

武汉大学化学与分子科学学院，武汉 430072

摘　要：

RNA是维持细胞生命活动最重要的基础物质之一，越来越多的证据表明它在细胞的生长发育中起重要的调控作用，成为生命科学中兴起的热点。然而，RNA存在时间短且不稳定的特点，造成RNA研究的困难。RNA的空间分布、转录调控机制以及RNA和其他生物大分子的相互作用都需要有效的标记分析方
法。随着化学生物学的发展，越来越多的RNA化学标记技术被开发出来，帮助人们更深入地研究RNA相关功能。该文主要介绍RNA体外标记技术、RNA代谢标记以及RNA邻近相互作用等方面的发展及应用。

 

通讯作者：翁小成 , Email:xcweng@whu.edu.cn 周　翔 , Email:xzhou@whu.edu.cn

RNA chemical labeling technology 

FANG Zhen-Tian , LU Zi-Ang , FANG Xin , WENG Xiao-Cheng^* , ZHOU Xiang^*

College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China

Abstract:

RNA has become a hot spot in life sciences as one of the most important basic substances for maintainingcell life to regulate cell growth. However, the features of RNA of short life and easy degradation make it difficult for RNA research. Researchers are looking for more effective labeling analysis methods to delve into the distribution of RNA in three-dimensional space, the transcriptional regulation mechanism of RNA and the interaction between RNA and biomacromolecule. With the development of chemical biology, more and more RNA chemical labeling technologies have been developed to help people study RNA-related functions more deeply. This review mainly introduces the development and application of RNA labeling technology in vitro, RNA metabolic labeling, and RNA proximity interactions.

Communication Author：WENG Xiao-Cheng , Email:xcweng@whu.edu.cn ZHOU Xiang , Email:xzhou@whu.edu.cn