《生命科学》 2021, 33(3): 312-325

细胞RNA影像解析技术的研究进展

陈显军 , 左方婷 , 谢　鑫 , 方梦悦 , 杨　弋^*

华东理工大学生物反应器工程国家重点实验室，上海 200237

摘　要：

RNA具有独特的三维结构和种类繁多的生物学功能，广泛参与细胞的各类生命活动，与多种疾病的发生与发展密切相关。长期以来，科学家们一直在追求可以对细胞中RNA的丰度和分布进行解析的技术。该文将对多种细胞RNA影像解析技术的研究进展进行介绍，并将对最有希望成为活细胞RNA理想标记成像技术的荧光RNA进行重点介绍，包括它的种类以及在活细胞RNA成像中的应用，同时还将探讨细胞RNA影像解析技术未来的发展方向以及面临的挑战。

 

通讯作者：杨　弋 , Email:yiyang@ecust.edu.cn

The advances in cellular RNA imaging technology 

CHEN Xian-Jun , ZUO Fang-Ting , XIE Xin , FANG Meng-Yue , YANG Yi^*

State Key Laboratory of Bioreactor Engineering, East China University of

Abstract:

RNAs exhibit unique three-dimensional structures and a variety of biological functions, and have been widely involved in diverse life activities and closely related to the occurrence and development of many diseases. During these years, scientists have been persuing technologies that can analyze the abundance and distribution of
RNA in cells. In this review, we describe the recent advances in cellular RNA  imaging technology, and focus on fluorescent RNA, which is the most promising tool as an ideal technology for RNA imaging in live cells, including its types and applications in RNA imaging, and meanwhile we discuss the direction and challenge of future development of cellular RNA imaging technology.

 

Communication Author：YANG Yi , Email:yiyang@ecust.edu.cn