《生命科学》 2021, 33(3): 303-311

RNA结合蛋白研究技术

陈冠霖 , 王怡旻 , 范　静 , 陈素丽 , 王建姝 , 王常收 , 张　力 , 徐　林 , 朱　怡 , 张　强 , 童　登 , 程　红^*

中国科学院分子细胞科学卓越创新中心，上海 200031

摘　要：

RNA结合蛋白在细胞中与RNA互作，广泛参与RNA的转录、加工、降解和出核转运等众多过程，从而调控RNA的生物学功能。随着RNA功能研究的逐渐深入，理解RNA与特定RNA结合蛋白的具体作用机制变得尤为重要，由此推动了RNA结合蛋白与RNA互作研究技术的迅猛发展。该文对经典常用的RNA结合蛋白与RNA互作研究技术进行综述，并根据其纯化中心(蛋白质或RNA)将其分成两大类，主要介绍相应的技术原理、应用领域及研究进展，并分析相关技术存在的问题，为RNA相关领域的研究提供参考。

 

通讯作者：程　红 , Email:hcheng@sibcb.ac.cn

Technologies of studying RNA binding proteins 

CHEN Guan-Lin , WANG Yi-Min , FAN Jing , CHEN Su-Li , WANG Jian-Shu , WANG Chang-Shou , ZHANG Li , XU Lin , ZHU Yi , ZHANG Qiang , TONG Deng , CHENG Hong^*

Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai 200031, China

Abstract:

RNA-binding proteins are widely involved in many biological processes by interacting with cellular RNA, such as RNA transcription, processing, degradation, and nuclear export, and thus play key roles in regulating the biological functions of targeted RNA. With the deepening understanding of RNA functions, it is particularly important to explain the mechanisms of the interaction between RNAs and RNA binding proteins, which promotes the rapid development of technologies about studying RNA binding protein-RNA interaction. This review
summarizes the classical and commonly used technologies in RNA binding protein-RNA interaction research, which are divided into two major categories based on different purification centers, protein or RNA. We also introduce their technical principles, application fields and research progress, and analyze the shortages of related technologies. This review will provide important references for RNA-related researches.

 

Communication Author：CHENG Hong , Email:hcheng@sibcb.ac.cn