《生命科学》 2021, 33(3): 292-302

基于高通量测序的RNA修饰检测技术

朱文煜¹ , 李蔡涛^1,2 , 杨文清¹ , 李　静¹ , 刘如娟^1,*

¹上海科技大学生命科学与技术学院，上海 201210 ²中国科学院分子细胞科学卓越创新中心，上海 200031

摘　要：

转录后修饰广泛存在于各种RNA分子中，对RNA发挥功能至关重要。目前，RNA上的化学修饰已达到160余种(https://iimcb.genesilico.pl/modomics/)，其中甲基化修饰是最常见的修饰类型。薄层层析、高效液相色谱及质谱等传统的检测方法对RNA修饰的鉴定和定量做出了重要贡献。然而，RNA修饰的精确定位以及对低丰度RNA分子的修饰检测等则依赖于近些年发展的适用于修饰检测的高通量测序技术。《生命科学》2018年发表的“RNA修饰检测技术”一文已对一些传统的RNA检测方法和部分高通量测序检测方法进行了系统的介绍。在此基础上，该文整理了目前常见的基于高通量测序的RNA修饰检测技术，并对其工作
原理和应用展开介绍。

 

通讯作者：刘如娟 , Email:liurj@shanghaitech.edu.cn

The detection methods of RNA modifications based on high-throughput sequencing

ZHU Wen-Yu¹ , LI Cai-Tao^1,2 , YANG Wen-Qing¹ , LI Jing¹ , LIU Ru-Juan^1,*

¹School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China ²Center for Excellence in Molecular Cell Science, CAS, Shanghai 200031, China

Abstract:

Post-transcriptional modifications widely exist in various RNA molecules and are essential for RNA function. At present, there are more than 160 kinds of chemical modifications on RNA (https://iimcb.genesilico.pl/modomics/), and methylation is the most common type of modification. Traditional RNA detection methods, such as thin layer chromatography, high performance liquid chromatography and mass spectrometry, have made important contributions to the identification and quantification of RNA modifications. However, the precise identification of RNA modifications and the detection of modifications on low abundant RNA molecules rely on some high-throughput sequencing based techniques, which have been developed in recent years. Traditional RNA detection methods and some high-throughput sequencing based methods have been introduced in the article “The detection methods of RNA modifications” published in this journal in 2018. Here, we summarize the popular detection methods of RNA modification based on high-throughput sequencing, and introduce their working principles and application.

Communication Author：LIU Ru-Juan , Email:liurj@shanghaitech.edu.cn