《生命科学》 2021, 33(3): 267-280

基于高通量测序的RNA信息解析技术

黄钧鸿 , 黄巧娟 , 李　斌 , 杨建华^*

中山大学生命科学学院，广州 510275

摘　要：

非编码RNA (noncoding RNA, ncRNA) 占据真核生物转录组的绝大部分，在各种生理和病理过程中发挥重要作用。随着高通量测序技术的发展，人们利用RNA 信息学技术解析到越来越多的非编码RNA的信息，并逐渐揭示其功能和作用机制。该文主要介绍非编码RNA 及其靶标鉴定、RNA 功能网络、RNA与蛋白质互作、RNA 修饰及RNA 二级结构的信息解析技术。

 

通讯作者：杨建华 , Email:yangjh7@mail.sysu.edu.cn

Bioinformatic methods for analyzing noncoding RNAs from high-throughput sequencing data 

HUANG Jun-Hong , HUANG Qiao-Juan , LI Bin , YANG Jian-Hua^*

School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China

Abstract:

Non-coding RNAs (ncRNAs) occupy most of the eukaryotic transcriptome, and play an important role in physiological and pathological processes. With the development of high-throughput sequencing technology, people have developed various bioinformatic tools and databases to analyze ncRNAs, and gradually revealed their regulatory functions and mechanisms. This review mainly introduces how to identify ncRNAs and their biological targets, regulatory networks of RNAs, interactions between RNAs and proteins, RNA modification as well as RNA secondary structures.

 

Communication Author：YANG Jian-Hua , Email:yangjh7@mail.sysu.edu.cn