《生命科学》 2021, 33(2): 255-264

数字PCR在生物学检测中应用的研究进展

黄　瑾¹ , 梁涛波² , 许恒毅^1,*

¹南昌大学食品科学与技术国家重点实验室，南昌 330047 ²江西省食品检验检测研究院，南昌 330001

摘　要：

数字PCR (digital PCR, dPCR)是在普通PCR和定量PCR基础上发展的第三代PCR技术，通过有限稀释和泊松分布统计实现精确的绝对定量检测。相比于前两代PCR技术，dPCR能够实现DNA模板的绝对定量且对PCR抑制剂具有更强的耐受性。目前，该技术在致病菌和病毒、基因突变、甲基化DNA、转基因成分和食品掺假的检测中都得到了广泛的应用。该文介绍了dPCR的原理并综述了其在生物学检测中应用的研究进展，以期为基于dPCR的检测方法的开发和应用提供参考和依据。

通讯作者：许恒毅 , Email:kidyxu@163.com

Research progress of application of digital PCR in biological detection

HUANG Jin¹ , LIANG Tao-Bo² , XU Heng-Yi^1,*

¹State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China ²Jiangxi Institute of Food Inspection and Testing, Nanchang 330001, China

Abstract:

Digital PCR (dPCR) is a third-generation PCR technology based on conventional PCR and quantitative PCR, which applies finite dilution and poisson statistics to achieve accurate absolute quantitative detection. Compared to the previous two generations of PCR technology, dPCR can achieve absolute quantification of DNA template and shows better tolerance to PCR inhibitors. At present, dPCR has been widely used in detection of pathogenic bacteria, viruses, genetic mutations, methylated DNA, genetically modified ingredients and food adulteration. This review introduces the principle of dPCR, summarizes its progress in biological detection area, with the aim to provide references for development and further application of dPCR.

Communication Author：XU Heng-Yi , Email:kidyxu@163.com