《生命科学》 2020, 32(11): 1176-1184

氨基肽酶N的结构与功能研究进展

连思琪^1,2 , 赵 静^1,2 , 刘家奇^1,2 , 朱国强^1,2,* , 夏芃芃^1,2,*

¹扬州大学兽医学院，比较医学研究院，扬州 225009 ²江苏省动物重要疫病与人兽共患病 防控协同创新中心，教育部农业与农产品安全国际合作联合实验室，扬州 225009

摘　要：

氨基肽酶N (APN) 是一种含有Zn²⁺ 的膜结合型外肽酶，属于金属蛋白酶M1 家族，相对分子质量约为150 kDa，在自然界广泛存在，有膜结合型和可溶型两种形式。作为一种“兼职酶”，APN 通过酶依赖性和非酶依赖性途径在哺乳动物体内参与多种生理病理活动。该文简述了APN 的发现和分类，以及哺乳动物APN 的晶体结构和胞外区的三种构象变化，并从肿瘤标志物、病原受体和免疫三个方面对APN 的主要功能进行分类汇总，旨在解析APN 的相关病理机制，为靶向APN 的疾病治疗及药物开发方案提供基础资料。

通讯作者：朱国强 , Email:yzgqzhu@yzu.edu.cn 夏芃芃 , Email:ppxia@yzu.edu.cn

Recent progresses on the structure and functions of aminopeptidase N

LIAN Si-Qi^1,2 , ZHAO Jing^1,2 , LIU Jia-Qi^1,2 , ZHU Guo-Qiang^1,2,* , XIA Peng-Peng^1,2,*

¹Institute of Comparative Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China ²Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China

Abstract:

Aminopeptidase N is a kind of membrane-bound exopeptidase which contains Zn²⁺ and belongs to the M1 family of metalloproteinases, about 150 kDa, widespread existing in nature. As a moonlighting enzyme, APN participates in a variety of physiological and pathological activities in mammals via both enzyme-dependent and -independent pathways. This paper briefly describes the discovery and classification of APN, as well as the crystal structure of mammalian APN and three conformational changes in their extracellular region. The main function of APN are summarized and classified based on three aspects: tumor markers, pathogenic receptors and immunity, aiming to analyze the underlying pathological mechanisms of APN and provide basic data for disease treatment and drug development targeting APN.

Communication Author：ZHU Guo-Qiang , Email:yzgqzhu@yzu.edu.cn XIA Peng-Peng , Email:ppxia@yzu.edu.cn