《生命科学》 2019, 31(9): 959-967

珍稀药用植物铁皮石斛的组学及功能基因研究进展

李以格，杨　杭，姜琪梦，陈研硕，王晓锋，陈　勇*

(温州大学生命与环境科学学院，温州 325035)

摘　要：摘　要：铁皮石斛为兰科珍稀名贵药用植物，具有极高的医药保健和生态观赏价值。以“高通量、高精度、低成本”为特点的新一代测序技术平台，高效推动了铁皮石斛基因组和转录组等组学基础研究，有关铁皮石斛多糖和石斛碱积累、生长发育调控、环境适应性等机制逐渐获得了分子数据支持。该文综述了近年来铁皮石斛基因组、转录组、蛋白质组等组学以及功能基因等研究，内容涉及其非生物胁迫、多糖合成、种子萌发等过程，最后对当前铁皮石斛分子生物学研究进行了讨论。

Investigation on omics and functional genes of Dendrobium officinale (Orchidaceae)， a precious medicinal herb

LI Yi-Ge， YANG Hang， JIANG Qi-Meng， CHEN Yan-Shuo， WANG Xiao-Feng， CHEN Yong*

(Life Science and Environmental College， Wenzhou University， Wenzhou 325035， China)

Abstract: Abstract: Dendrobium officinale Kimura et Migo (Orchidaceae) is an important， traditional Chinese herb with high medicinal， healthy as well as ecological， ornamental value. Next-generation sequencing platform， characteristic of remarkable technique advantages with high throughput， high precision as well as low cost， provided robust support for rapid understanding of omics knowledges on genome and transcriptome etc. of D.officinale. Therefore， molecular mechanisms regarding polysaccharides accumulation， development regulation and environment adaptation of D.officinale came to be elucidated. In this study， we review the dynamic progress on genome， transcriptome， proteome etc. as well as functional genes associated with abiotic stress response， polysaccharide biosynthesis and seeds germination etc. in D.officinale， and also discuss the existing problems on molecular investigation of D. officinale.