《生命科学》 2019, 31(6): 527-533

链霉菌调控因子bldA的作用机制研究进展

范佳奕1，2，王恩多1，2，3*

(1 中国科学院上海生物化学与细胞生物学研究所，分子细胞科学卓越创新中心，分子生物学国家重点实验室，上海 200031；2 中国科学院大学，北京 100864；3 上海科技大学生命科学与技术学院，上海 201210)

摘　要：摘　要：链霉菌是一类具有特殊的形态分化和次生代谢的放线菌。bldA 编码链霉菌中唯一能有效识别亮氨酸密码子UUA 的tRNALeu，是链霉菌次生代谢途径的必需基因，目前对于其全局调控功能已有详细报道。bldA 突变或缺失会导致链霉菌不能完成气生菌丝分化和抗生素合成，这种调控主要是在翻译水平上完成。UUA 密码子缺少有效的tRNA 翻译，导致mRNA 翻译受影响。bldA 自身也受到其下游靶基因的负反馈调控。此外，bldA 还有很多尚不明确的调控机制，回答这些问题能帮助我们更好地理解抗生素合成途径，为构建有应用前景的菌株奠定理论基础。

Advance of mechanism of the regulator bldA in Streptomyces

FAN Jia-Yi1，2， WANG En-Duo1，2，3*

(1 State Key Laboratory of Molecular Biology， CAS Center for Excellence in Molecular Cell Science， Shanghai Institute of Biochemistry and Cell Biology， Chinese Academy of Sciences， Shanghai 200031， China; 2 University of Chinese Academy of Sciences， Beijing 100864， China; 3 School of Life Science and Technology， Shanghai Tech University， Shanghai 201210， China)

Abstract: Abstract: Streptomyces is a genus of actinobacteria characterized by morphological differentiation and secondary metabolism. bldA in Streptomyces encodes the only tRNA for a rare leucine UUA， which is necessary for secondary
    metabolism. So far there have been detailed reports about how bldA works as a global regulator. Knockout or mutation on bldA led to failure of production of aerial hyphae and antibiotics. Which is attributed to the regulatory effect of bldA on the translation level， as mRNA translation is disrupted caused by the lack of an effective tRNALeu. bldA also receives negative feedback signal from other genes. Besides， there are still unclear regulating mechanism of bldA. Solving this question will provide us with a better understanding on antibiotics synthesis and establish a theoretical foundation for construction of promising strains.