《生命科学》 2019, 31(4): 413-422

合成生物学驱动的智能生物制造研究进展

王文方，钟建江*

(上海交通大学生命科学技术学院，微生物代谢国家重点实验室，教育部代谢与发育科学国际合作联合实验室，上海 200240)

摘　要：摘　要：合成生物学的迅猛发展给包括药物和化学品在内的生物制造带来了强劲动力。它助力生物合成关键元件的挖掘，丰富了智能生物制造所必需的基础( 催化) 元件库；底盘细胞的性能优化为高效生物制造奠定了基石和平台。合成生物学经典的“设计- 构建- 测试- 学习”则是创建高效智能细胞工厂的核心研发内容。天然宿主的系统代谢工程和合成生物学以及无细胞体系的体外合成生物学，是实现高效生物制造和替代底盘细胞体系的可选途径。该文简要综述近年国内外的相关研究进展。

Recent advances in smart biomanufacturing driven by synthetic biology

WANG Wen-Fang， ZHONG Jian-Jiang*

(State Key Laboratory of Microbial Metabolism， School of Life Sciences and Biotechnology， Shanghai Jiao Tong University， Shanghai 200240， China)

Abstract: Abstract: The rapid development of synthetic biology has given strong impetus to biological manufacturing，including drugs and chemicals. It helps to mine the key elements of biosynthesis， enriches the essential basic (catalytic) element library for intelligent biological manufacturing， and optimizes the performance of chassis cells， which lays the foundation and platform for efficient biological manufacturing. The classic “design-build-testlearning”of synthetic biology is central to the creation of efficient smart cell factories. Systematic metabolic
    engineering and biosynthetic biology of natural hosts as well as in vitro cell-free biosynthetic systems are alternative approaches to efficient biological production and replacement of chassis cell systems. This article briefly reviews
    the research progress at home and abroad in recent years.