水稻全基因组选择育种技术平台构建与应用
邱树青1,2,陆 青1,2,喻辉辉1,倪雪梅3,4,张耕耘3,4,何 航5,谢为博6,周发松1,2*
(1 中国种子集团有限公司生命科学技术中心,武汉 430206;2 武汉双绿源创芯科技研究院,武汉 430056;3 深圳华大生命科学研究院,深圳 518083;4 农业基因组学国家重点实验室,深圳 518083;5 北京大学蛋白质与植物基因研究国家重点实验室,北京 100871;6 华中农业大学作物遗传改良国家重点实验室,武汉 430070)

摘 要:摘 要:随着DNA 标记技术的发展和越来越多的功能基因被鉴定和克隆,水稻全基因组选择育种已经成为精准高效的新品种培育方法。该文重点介绍新创建的基于高通量测序和基因组育种芯片的全基因组选择技术平台,以及建立的水稻基因组序列变异数据库、受选择功能区段鉴定技术体系和品种系谱溯源技术体系。这些技术平台和体系将有助于水稻全基因组选择育种工作的开展,提高育种效率,更好地满足消费市场对品种培育提出的新要求。

The development and application of rice whole genome selection breeding platform
QIU Shu-Qing1,2, LU Qing1,2, YU Hui-Hui1, NI Xue-Mei3,4, ZHANG Geng-Yun3,4, HE Hang5, XIE Wei-Bo6, ZHOU Fa-Song1,2*
(1 Life Science and Technology Center, China National Seed Group Co., Ltd, Wuhan 430206, China; 2 Wuhan Greenfafa Institute of Novel Genechip R&D, Wuhan 430056, China; 3 BGI-Shenzhen, Shenzhen 518083, China; 4 State Key Laboratory of Agricultural Genomics, Shenzhen 518083, China; 5 State Key Laboratory of Protein and Plant Gene Research, Peking University, Beijing 100871, China; 6 National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China)

Abstract: Abstract: It is becoming a practical method to develop super green rice varieties efficiently through whole genome selections with DNA marker technology ready to practise as well as more and more rice functional genes being identified and cloned. In this paper, we presented two whole genome selection methods which were established based on high throughput sequencing technology and whole genome rice gene-chip. We also built RiceVerMap database, a functional haplotype identification system, and a rice pedigree analysis system. All of these may greatly help rice breeders to perform the whole genome selection method in their breeding programs to better meet consumers’ novel demand for safety and high quality with more efficient development of new rice varieties.

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